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产品名称:P0162/pCDNA3.1-Myc-HisB(-)哺乳表达质粒
启动子: CMV
复制子: pUC ori,F1 ori
终止子: BGH poly(A)signal
质粒分类: 哺乳细胞载体;蛋白过表达质粒
质粒大小: 5520bp
质粒标签: 6X His, myc
原核抗性: 氨苄青霉素Amp
筛选标记: 新霉素Neo
克隆菌株: DH5α
培养条件: 37℃,有氧,LB
表达宿主: 哺乳细胞
特点:是染色质外的双链共价闭合环形DNA(covalently closed circular DNA,cccDNA),可自然形成超螺旋结构,不同质粒大小在2-300kb之间,<15kb的小质粒比较容易分离纯化,>15kb的大质粒则不易提取。
简介:pcDNA3.1/myc-His(-) A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. The vectors are supplied in three reading frames to facilitate in frame cloning, with a C-terminal peptide containing a polyhistidine metal-binding tag and the myc (c-myc) epitope. The human cytomegalovirus immediate-early (CMV) promoter provides highlevel expression in a wide range of mammalian cells. In addition, the vector will replicate episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and nonreplicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/myc-His(-)/lacZ, is the pcDNA3.1/myc-His(-) A vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. It is included for use as a positive control for transfection, expression, and detection in the cell line of choice.
P0162/pCDNA3.1-Myc-HisB(-)哺乳表达质粒 质粒分类:
1.根据质粒能否通过细菌的接合作用,可分为接合性质粒和非接合性质粒。接合性质粒带有与接合传递有关的基因。非接合质粒在一定条件下通过与其共存的接合质粒的诱动或转导而传递。
2.根据质粒的不相容性,可分为不相容性和相容性。不相容性指结构相似、密切相关的质粒不能稳定地共存于同一宿主细菌内的现象,反之为相容性。常用于流行病学的调查。
3.根据质粒在细菌内的复制类型可分为两类:严紧控制型和松弛控制型。严紧控制复制型质粒的复制酶系与染色体DNA复制共用,只能在细胞周期的一定阶段进行复制,当细胞染色体停止复制时,质粒也就不再复制。松弛控制复制型的质粒的复制酶系不受染色体DNA复制酶系的影响,在整个细胞生长周期中随时都可以复制,在染色体复制已经停止时质粒仍能继续复制。
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