流感抗原快速检测试剂盒

日本富士 流感抗原快速检测试剂盒

广州健仑生物科技有限公司

广州健仑长期供应各种流感检测试剂，包括进口和国产的品牌，主要包括日本富士瑞必欧、日本生研、美国BD、美国NovaBios、美国binaxNOW、英国clearview、广州创仑等主流品牌。

主要检测：甲型流感病毒检测试剂、乙型流感病毒检测试剂、甲乙型流感病毒检测试剂、A+B流感病毒检测试剂盒、流感病毒抗原快速检测卡、流感病毒抗体快速检测试剂盒、流感快速检测试剂 c1c2。

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

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日本富士 流感抗原快速检测试剂盒

【产品说明书】

【原理】 

流感病毒检测卡以双抗体夹心法为基础，采用免疫层析金标记技术，快速检测流感病毒。  

【试剂组成】 

1. 流感病毒快速检测卡40片/盒    

2. 样本稀释液40管/盒      

3. 棉签40支/盒  

【操作方法】 一、样品制备： 

1. 本检测卡采集样品为眼、气管分泌物。将棉签插入分泌物zui多的部位，轻轻摇动棉签，让棉签充分吸收分泌物。 

2. 将棉签在稀释液中充分搅拌并反复挤压试管壁，让分泌物充分溶解到稀释液中，得到待检样品。 

3. 样品一般须当即进行检测，否则应冷藏保存，超过24小时的，应该冷冻保存。  

二、操作步骤： 

1. 使用前将试剂盒和样品恢复至室温。 

2. 将棉签浸入装有样品稀释液的试管，充分搅拌混匀后，用一次性滴管取上清液。

3. 取出检测卡，开封后平放于桌面上，从滴管中缓慢而准确地逐滴加入2–3滴混合液。 

4. 加样品液后，约30秒内，红色的液体从靠样品孔的观察窗边缘涌出。朝另一方向流动。 

5. 五分钟后判断结果，超过三十分钟的结果判读无效。  

【结果判定】        

1. 阳性：在观察孔内，若对照线显色，检测线同时显色，判为阳性(如图一、图二)。阳性结果表明检测液

中含有流感病毒。 

2. 阴性：在观察孔内，若对照线显色，而检测线不显色，判为阴性（如图三）。阴性结果表明检测液中不含流感病毒。 

3. 无效：在观察孔内，若对照线不显色，则结果无效，建议重新测试(如图四、图五)。   

【产品介绍】

日本富士 

⑴Real-time RT-PCR
ORF1/ORF2区是诺如病毒基因组中zui保守的区域，在同一基因型不同毒株间有相同的保守序列，根据这段保守区域可用于设计TaqMan为基础的Real-time RT-PCR的引物和探针。除可检测病例临床标本中的诺如病毒RNA外，引物和探针经优化提高灵敏度后，还可用于环境样本（如食物和水）的检测。Real-time RT-PCR的敏感性高于传统RT-PCR，可检测诺如病毒G I群和G II群。核酸检测试剂盒 
⑵传统RT-PCR
采用传统RT-PCR对Real-time RT-PCR阳性标本的PCR产物进行测序，通过序列分析确定诺如病毒的基因型。测定ORF2完整衣壳蛋白基因序列，是诺如病毒基因分型的金标准。基因组中四种不同的区域（Region A-D）均可用于诺如病毒基因分型，基于衣壳蛋白区Region C和D两区域分型效果更好。但对于GII.4变异株的进一步分型，仅根据Region C序列不足以区别，需进一步扩增Region D。核酸检测试剂盒 
2.抗原检测
由于诺如病毒抗原高度变异（基因型超过29个）且某些基因型存在抗原漂移（如GII.4型），开发广泛反应的ELISA方法存在较大挑战。虽然目前已有商品化的试剂盒如IDEIA（Oxiod,英国）、SRSV(Ⅱ)-AD（Denka Seiken,日本）以及RIDASCREEN（r-Biopharm AG，德国），但其敏感性（36%-80%）和特异性（47%-*）均不太理想。即使已通过美国FDA认证的RIDASCREEN诺如病毒第三代ELISA试剂盒，也没有在美国广泛应用。ELISA可适用于暴发疫情中大量样本的筛查，但不适合散发病例的检测。ELISA检测结果阴性的样本还需要通过Real-time RT-PCR进行第二次检验确认。鉴于ELISA试剂盒成本高、敏感性低，ELISA方法仅可作为辅助检测手段核酸检测试剂盒  。
九、预防控制措施核酸检测试剂盒
目前，针对诺如病毒尚无特异的抗病PCR检测试剂盒和疫苗，其预防控制主要采用非药物性预防措施，包括病例管理、手卫生、环境消毒、食品和水安全管理、风险评估和健康教育。这些措施既适用于聚集性和暴发疫情的处置，也适用于散发病例的预防控制。核酸检测试剂盒 
（一）病例管理
鉴于诺如病毒的高度传染性，对诺如病毒感染人员进行规范管理是阻断传播和减少环境污染的有效控制手段。原则如下：
1. 病例：在其急性期至症状*消失后72小时应进行隔离。轻症患者可居家或在疫情发生机构就地隔离；症状重者需送医疗机构按肠道传染病进行隔离治疗，医疗机构应做好感染控制，防止院内传播。
2. 隐PCR检测试剂盒染者：建议自诺如病毒核酸检测阳性后72小时内进行居家隔离。

日本富士 

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、食品安全、化妆品检测、药物滥用检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

想了解更多的产品及服务请扫描下方二维码：

【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

【】 
【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

⑴Real-time RT-PCR
The ORF1 / ORF2 region is the most conserved region in the Norovirus genome and shares the same conserved sequence among different strains of the same genotype, allowing for the design of TaqMan-based Real-time RT-PCR primers based on this conserved region Probe. In addition to detecting Norovirus RNA in clinical specimens, primers and probes are optimized for sensitivity and can be used for the detection of environmental samples such as food and water. Real-time RT-PCR is more sensitive than traditional RT-PCR in detecting Norovirus G I and G II populations. Nucleic acid test kit
⑵ traditional RT-PCR
Real-time RT-PCR positive samples were sequenced by conventional RT-PCR, and the norovirus genotypes were determined by sequence analysis. Determination of ORF2 complete capsid protein gene sequence is the norovirus genotyping gold standard. Four different regions of the genome (Region A-D) can be used for genotyping norovirus, and the classification based on Region C and D of the capsid protein region is better. However, for the further typing of GII.4 mutants, Region D is not enough to distinguish only based on the Region C sequence, and Region D needs to be further amplified. Nucleic acid test kit
Antigen detection
Due to the high variation of Norovirus antigens (more than 29 genotypes) and the presence of antigenic drift in some genotypes (eg, GII.4 type), there is a big challenge in developing a widely-reaction ELISA approach. Although commercial kits such as IDEIA (Oxiod, UK), SRSV (II) -AD (Denka Seiken, Japan) and RIDASCREEN (r- Biopharm AG, Germany) are available, their sensitivity (36-80% ) And specificity (47% -*) were less than ideal. Even though it has passed the US FDA RIDASCREEN Norovirus third-generation ELISA kit, it has not been widely used in the United States. ELISA can be applied to a large number of outbreaks of the screening of the epidemic, but not suitable for detection of sporadic cases. Negative ELISA test samples also need to be confirmed by Real-time RT-PCR for the second test. In view of the high cost and low sensitivity of ELISA kits, the ELISA method can only be used as an aid in the detection of nucleic acid kits.
Nine, prevention and control measures Nucleic acid test kit
At present, there are no specific resistance PCR test kits and vaccines against Norovirus, and their prevention and control mainly use non-drug preventive measures including case management, hand hygiene, environmental disinfection, food and water safety management, risk assessment and health education. These measures apply both to the handling of clusters and outbreaks and to the prevention and control of sporadic cases. Nucleic acid test kit
(A) case management
In view of the high contagion of Norovirus, the normative management of Norovirus infected individuals is an effective means of controlling transmission and reducing environmental pollution. The principle is as follows:
1. Case: Isolation should be performed 72 hours after its acute phase has compley disappeared. Slight patients may be home or place of origin in the epidemic of isolation; severe symptoms need to be sent to medical institutions in accordance with intestinal infectious diseases isolation and treatment, medical institutions should be good infection control to prevent the spread of the hospital.
2. Implicit PCR detection kit Infected: It is recommended to self-Norovirus nucleic acid test positive for 72 hours after the home isolation.