产品详情
HCT-116细胞, 低分化结肠腺癌细胞
A34351 人孤腓肽(OFQ/N)ELISA试剂盒 Human orphanin FQ/nociceptin,OFQ/N ELISA Kit 96T/48T
A34352 人基质裂解素(ST1)ELISA试剂盒 Human Stromelysin-1,ST1 ELISA Kit 96T/48T
A34353 人基质裂解素(ST2)ELISA试剂盒 Human Stromelysin-2,ST2 ELISA Kit 96T/48T
A34354 人基质裂解素(ST3)ELISA试剂盒 Human Stromelysin-3,ST3 ELISA Kit 96T/48T
A34355 人脂氧素A4(LXA4)ELISA试剂盒 Human Lipoxin A4,LXA4 ELISA Kit 96T/48T
A34356 人蛋白酶3特异性抗中性粒细胞胞质抗体HCT-116细胞, 低分化结肠腺癌细胞(PR3-ANCA)ELISA试剂盒 Human proteinase-antineutrophil cytoplasmic antibody,PR3-ANCA ELISA Kit 96T/48T
A34357 人β-促脂素(β-LPH)ELISA试剂盒 Human β-lipotropic hormone,β-LPH ELISA Kit 96T/48T
A34358 人脂磷壁酸(LTA)ELISA试剂盒 Human lipoteichoic acids,LTA ELISA Kit 96T/48T
A34359 人乙胺碘呋酮(AD)ELISA试剂盒 Human amiodarone,AD ELISA Kit 96T/48T
A34360 人唾液酸(SA)ELISA试剂盒 Human Sialic acid,SA ELISA Kit 96T/48T
初代HCT-116细胞, 低分化结肠腺癌细胞消化培养法
1. 准备:取各种已消毒的培养用品置于净化台面,紫外线消毒20分钟。开始工作前先洗手、75%酒精擦拭手至肘部。
2. 布局:点燃酒精灯,安装吸管帽。
3. 处理组织:把组织块置于烧杯中,用Hanks液漂洗2~3次,去除血污;如怀疑组织可能污染,可先置于含有青链霉素的混合液中30~60分钟。
4. 剪切:用眼科剪把组织切成2~3毫米大小的块,以便于消化。加入比组织块总量多30~50倍的胰蛋白酶液,然后一并倒入三角烧瓶中,结扎瓶口或塞以胶塞。
5. 消化:或用恒温水浴,或置于37℃温箱消化均可,消化中每隔20分钟应摇动一次,如用电磁恒温搅拌器消化更好。消化时间依组织块的大小和组织的硬度而定。
6. 分离:在消化过程中见消化液发混浊时,可用吸管吸出少许消化液在镜下观察,如组织已分散成细胞团或单个细胞,立即终止消化,随即通过适宜不锈钢筛,滤掉尚未充分消化开的组织块。低速(500~1000转/分)离心消化液5分钟,吸出上清,加入适量含有血清的培养液。
7. 计数:用计数板计数,如细胞悬液细胞密度过大,再补加培养液调整后,分装入培养瓶中。对大多数细胞来说,pH要求在7.2~7.4范围,培养液呈微红色,如颜色偏黄,说明液体变酸,可用NaHCO3调整。
8. 培养:置于36.5℃温箱培养,如用CO2温箱培养,瓶口需用纱布棉塞或螺旋帽堵塞,纱布塞易生霉菌,每次换液时需要换新塞。
A34311 人假定蛋白LOC677340 ELISA试剂盒 human hypothetical protein LOC677340 ELISA Kit 96T/48T
A34312 人假定蛋白LOC433328 ELISA试剂盒 human hypothetical protein LOC433328 ELISA Kit 96T/48T
A34313 人髓样分化因子初次应答基因88(MYD88)ELISA试剂盒 human myeloid differentiation primary response gene 88,MYD88 ELISA Kit 96T/48T
A34314 人干扰素活化基因205(Ifi205)ELISA试剂盒 human interferon activated gene 205,Ifi205 ELISA Kit 96T/48T
A34315 人白细胞免疫球蛋白样受体亚家族B成员4(LILRB4)ELISA试剂盒 human leukocyte immunoglobulin-like receptor subfamily B member 4,LILRB4 ELISA Kit 96T/48T
A34316 人C型凝集素结构域家族4成员E(CLEC4E)ELISA试剂盒 human C-type lectin domain family 4 member e,CLEC4E ELISA Kit 96T/48T
A34317 人假定蛋白LOC677168 ELISA试剂盒 human Hypothetical protein LOC677168 ELISA Kit 96T/48T
A34318 人淋巴细胞抗原6复合物基因座A(Ly6a)ELISA试剂盒 human lymphocyte antigen 6 complex locus A,Ly6a ELISA Kit 96T/48T
A34319 人嗜酸性白血球相关之RNA水解酵素家族成员1(EAR1)ELISA试剂盒 human eosinophil-associated ribonuclease A family member 1,EAR1 ELISA Kit 96T/48T
A34320 人混合系列蛋白激酶样结构域(MLKL)ELISA试剂盒 human mixed lineage kinase domain-like,MLKL ELISA Kit 96T/48T
A34321 人过氧化物酶体增殖激活物受体γ辅激活因子1α(PPARGC1)ELISA试剂盒 human peroxisome proliferative activated receptor gama coactivator 1 alpha,PPARGC1 ELISA Kit 96T/48T
A34322 人类似cDNA顺序BC027382 ELISA试剂盒 human similar to cDNA sequence BC027382 ELISA Kit 96T/48T
A34323 人类似RIKEN cDNA 4931408D14 基因 ELISA试剂盒 human similar to RIKEN cDNA 4931408D14 gene ELISA Kit 96T/48T
A34324 人REST协阻抑因子3(RCOR3)ELISA试剂盒 human REST corepressor 3,RCOR3 ELISA Kit 96T/48T
A34325 人集蛋白亚家族成员11(COLEC11)ELISA试剂盒 Human collectin sub-family member 11,COLEC11 ELISA Kit 96T/48T
A34326 人α1β糖蛋白(α1β-GP)ELISA试剂盒 Human α1β glycoprotein,α1β-GP ELISA Kit 96T/48T
A34327 人可溶性髓系细胞触发受体-1(sTREM-1)ELISA试剂盒 Human soluble Triggering Receptor Expresses on Myeloid Cells-1,sTREM-1 ELISA Kit 96T/48T
A34328 人肌抑素(MSTN)ELISA试剂盒 Human Myostatin,MSTN ELISA Kit 96T/48T
A34329 人黑素瘤衍生亮氨酸拉链额外核因子(MLZE)ELISA试剂盒 Human melanomaderived leucine zipper extra-nuclear factor,MLZE ELISA Kit 96T/48T
A34330 人二磷酸尿核苷葡糖醛酸基转移酶2家族肽B4(UGT2B4)ELISA试剂盒 Human uridine dipho-sphate glucuronosyltransferase 2 family polypeptide B4,UGT2B4 ELISA Kit 96T/48T
A34331 人蛋白磷酸酶1调控/抑制因子亚基1A(PPP1R1A)ELISA试剂盒 Human protein phosphatase 1 regulatory subunit A,PPP1R1A ELISA Kit 96T/48T
A34332 人腺苷三磷酸结合盒转运体G2(ABCG2)ELISA试剂盒 Human ATP-binding cassette transporter G2,ABCG2 ELISA Kit 96T/48T
A34333 人鸟苷酸解离抑制因子(GDI)ELISA试剂盒 Human Guanine nucleotide dissociation Inhibitor,GDI ELISA Kit 96T/48T
A34334 人八聚体转录因子(OTF2B)ELISA试剂盒 Human octamer transcription factor 2B,OTF2B ELISA Kit 96T/48T
A34335 人八聚体转录因子(OTF2A)ELISA试剂盒 Human octamer transcription factor 2A,OTF2A ELISA Kit 96T/48T
A34336 人脱氧尿三磷酸(DUTP)ELISA试剂盒 Human deoxyuridinetriphate,DUTP ELISA Kit 96T/48T
A34337 人协同刺激分子受体(CMR)ELISA试剂盒 Human costimulatory molecules recptor,CMR ELISA Kit 96T/48T
A34338 人肽聚糖(PG)ELISA试剂盒 Human peptidoglycan,PG ELISA Kit 96T/48T
A34339 人脂阿拉伯甘露聚糖(LAM)ELISA试剂盒 Human Lipoarabinomannan,LAM ELISA Kit 96T/48T
A34340 人未甲基化寡聚脱氧核苷酸(CpG-ODN)ELISA试剂盒 Human CpG oligodeoxynucleotide,CpG-ODN ELISA Kit 96T/48T
A34341 人ICE蛋白酶激活因子(IRAP)ELISA试剂盒 Human ICE protease-activating factor,IRAP ELISA Kit 96T/48T
A34342 人非甲基化寡核苷酸(NON)ELISA试剂盒 Human nonmethylated oligonucleotide,NON ELISA Kit 96T/48T
A34343 人蛋白Z(Protein Z)ELISA试剂盒 Human Protein Z ELISA Kit 96T/48T
A34344 人蛋白S(Protein S)ELISA试剂盒 Human Protein S ELISA Kit 96T/48T
A34345 人可溶性血管内皮细胞蛋白C受体(sEPCR)ELISA试剂盒 Human soluble endothelial protein C receptor,sEPCR ELISA Kit 96T/48T
A34346 HCT-116细胞, 低分化结肠腺癌细胞人CCAAT增强子结合蛋白ε(C/EBPε)ELISA试剂盒 Human CCAAT/enhancer binding protein epsilon,C/EBPε ELISA Kit 96T/48T
A34347 人CCAAT增强子结合蛋白δ(C/EBPδ)ELISA试剂盒 Human CCAAT/enhancer binding protein deta,C/EBPδ ELISA Kit 96T/48T
A34348 人CCAAT增强子结合蛋白α(C/EBPα)ELISA试剂盒 Human CCAAT/enhancer binding protein alpha,C/EBPα ELISA Kit 96T/48T
A34349 人基膜聚糖/内腔蛋白(LUM)ELISA试剂盒 Human lumican,LUM ELISA Kit 96T/48T
A34350 人Na+/H+交换体3(NHE3)ELISA试剂盒 Human Na+/H+ exchanger 3,NHE3 ELISA Kit 96T/48T