产品详情
SKOV-3/DDP细胞, 卵巢癌顺铂耐药株
A34140 人非神经元性烯醇化酶(NNE)ELISA试剂盒 Human non-neuronal enolase,NNE ELISA Kit 96T/48T
A34141 人多巴胺-β羟化酶(DBH)ELISA试剂盒 Human dopamine-β-hydroxylase,DBH ELISA Kit 96T/48T
A34142 人胆碱乙酰化酶(CHAc)ELISA试剂盒 Human choline acetylase,CHAc ELISA Kit 96T/48T
A34143 人异柠檬酸脱氢酶(ICD)ELISA试剂盒 SKOV-3/DDP细胞, 卵巢癌顺铂耐药株Human isocitrate dehydrogenase,ICD ELISA Kit 96T/48T
A34144 人胰蛋白酶原Ⅱ(Try-Ⅱ)ELISA试剂盒 Human Trypsinogen-2,Try-ⅡELISA Kit 96T/48T
A34145 人腺苷脱氨酶(ADA)ELISA试剂盒 Human adenosine deaminase,ADA ELISA Kit 96T/48T
A34146 人天门冬氨酸氨基转移酶(AST)ELISA试剂盒 Human Aspartate aminotransferase,AST ELISA Kit 96T/48T
初代SKOV-3/DDP细胞, 卵巢癌顺铂耐药株消化培养法
1. 准备:取各种已消毒的培养用品置于净化台面,紫外线消毒20分钟。开始工作前先洗手、75%酒精擦拭手至肘部。
2. 布局:点燃酒精灯,安装吸管帽。
3. 处理组织:把组织块置于烧杯中,用Hanks液漂洗2~3次,去除血污;如怀疑组织可能污染,可先置于含有青链霉素的混合液中30~60分钟。
4. 剪切:用眼科剪把组织切成2~3毫米大小的块,以便于消化。加入比组织块总量多30~50倍的胰蛋白酶液,然后一并倒入三角烧瓶中,结扎瓶口或塞以胶塞。
5. 消化:或用恒温水浴,或置于37℃温箱消化均可,消化中每隔20分钟应摇动一次,如用电磁恒温搅拌器消化更好。消化时间依组织块的大小和组织的硬度而定。
6. 分离:在消化过程中见消化液发混浊时,可用吸管吸出少许消化液在镜下观察,如组织已分散成细胞团或单个细胞,立即终止消化,随即通过适宜不锈钢筛,滤掉尚未充分消化开的组织块。低速(500~1000转/分)离心消化液5分钟,吸出上清,加入适量含有血清的培养液。
7. 计数:用计数板计数,如细胞悬液细胞密度过大,再补加培养液调整后,分装入培养瓶中。对大多数细胞来说,pH要求在7.2~7.4范围,培养液呈微红色,如颜色偏黄,说明液体变酸,可用NaHCO3调整。
8. 培养:置于36.5℃温箱培养,如用CO2温箱培养,瓶口需用纱布棉塞或螺旋帽堵塞,纱布塞易生霉菌,每次换液时需要换新塞。
A34069 人3-羟基-3-甲基戊二酰辅酶A合酶1(HMGCS1)ELISA试剂盒 human 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1,HMGCS1 ELISA Kit 96T/48T
A34070 人嗜酸性白血球相关之RNA水解酵素家族成员12(EAR12)ELISA试剂盒 human eosinophil-associated ribonuclease A family member 12,EAR12 ELISA Kit 96T/48T
A34071 人嗜酸性白血球相关之RNA水解酵素家族成员3(EAR3)ELISA试剂盒 human eosinophil-associated ribonuclease A family member 3,EAR3 ELISA Kit 96T/48T
A34072 人嗜酸性白血球相关之RNA水解酵素家族成员2(EAR2)ELISA试剂盒 human eosinophil-associated ribonuclease A family member 2 ELISA Kit 96T/48T
A34073 人二氢嘧啶酶样3(DPYSL3)ELISA试剂盒 human dihydropyrimidinase-like 3,DPYSL3 ELISA Kit 96T/48T
A34074 人色氨酰tRNA合成酶(WARS)ELISA试剂盒 human tryptophanyl-tRNA synthetase,WARS ELISA Kit 96T/48T
A34075 人己糖激酶3(HK3)ELISA试剂盒 human hexokinase 3,HK3 ELISA Kit 96T/48T
A34076 人血小板型磷酸果糖激酶(PFKP)ELISA试剂盒 human phosphofructokinase-plaet,PFKP ELISA Kit 96T/48T
A34077 人尿苷二磷酸葡萄糖神经酰胺葡萄糖基转移酶(UGCG)ELISA试剂盒 human UDP-glucose ceramide glucosyltransferase ELISA Kit 96T/48T
A34078 人凋亡相关半胱氨酸肽酶4(Casp4)ELISA试剂盒 human caspase 4-apoptosis-related cysteine peptidase,Casp4 ELISA Kit 96T/48T
A34079 人吡哆醛/吡哆醇维生素B6激酶(PDXK)ELISA试剂盒 human caspase 4-apoptosis-related cysteine peptidase,Casp4 ELISA Kit 96T/48T
A34080 人二氢嘧啶酶样2(DPYSL2)ELISA试剂盒 human dihydropyrimidinase-like 2,DPYSL2 ELISA Kit 96T/48T
A34081 人血清/糖皮质激素调节激酶2(GSK2)ELISA试剂盒 human serum/glucocorticoid regulated kinase 2,GSK2 ISA KIT 96T/48T
A34082 人丝裂原活化蛋白激酶激酶6(MKK6)ELISA试剂盒 human mitogen activated protein kinase kinase 6,MKK6 ELISA Kit 96T/48T
A34083 人吡哆醛/吡哆醇维生素B6磷酸酶(PDXP)ELISA试剂盒 human pyridoxal/pyridoxine vitamin B6-phosphatase,PDXP ELISA Kit 96T/48T
A34084 人T细胞特异性鸟苷三磷酸酶(Tgtp)ELISA试剂盒 Human T-cell specific GTPase,Tgtp ELISA Kit 96T/48T
A34085 人法尼基二磷酸法尼基转移酶1(FDFT1)ELISA试剂盒 Human farnesyl-diphosphate farnesyltransferase 1,FDFT1 ELISA Kit 96T/48T
A34086 人D-乳酸脱氢酶(D-LDH) ELISA试剂盒 Human D-Lactate Dehydrogenase,D-LDH ELISA Kit 96T/48T
A34087 人α1抗胰蛋白酶(α1-AT)ELISA试剂盒 Human α1-Antitrypsin,α1-AT ELISA Kit 96T/48T
A34088 人激肽释放酶1(KLK 1)ELISA试剂盒 Human Kallikrein 1,KLK 1 ELISA Kit 96T/48T
A34089 人磷酸化乙酰辅酶A(PaCoA)ELISA试剂盒 Human Phosphorylated acetyl CoA,PaCoA ELISA Kit 96T/48T
A34090 人磷酸甘油酸变位酶2(PGAM2)ELISA试剂盒 Human phosphoglycerate mutase 2,PGAM2 ELISA Kit 96T/48T
A34091 人前列腺素D合成酶(PTGDS)ELISA试剂盒 Human Prostaglandin D synthase,PTGDS ELISA Kit 96T/48T
A34092 人肽酰脯氨酰异构酶(亲环蛋白)样1(PPIL1)ELISA试剂盒 Human peptidylprolyl isomerase(cyclophilin)-like 1,PPIL1 ELISA Kit 96T/48T
A34093 人促分裂素原活化蛋白激酶激活的蛋白激酶3(MAPKAPK3)ELISA试剂盒 Human mitogen-activated protein kinase-activated protein kinase 3,MAPKAPK3 ELISA Kit 96T/48T
A34094 人血管紧张素Ⅰ转化酶(ACEⅠ)ELISA试剂盒 Human Angiotensin Ⅰ Converting Enzyme,ACEⅠ ELISA Kit 96T/48T
A34095 人胱天蛋白酶12(Casp-12)ELISA试剂盒 Human Caspase 12,Casp-12 ELISA Kit 96T/48T
A34096 人蛋白激酶A(PKA)ELISA试剂盒 Human Protein kinase A,PKA ELISA Kit 96T/48T
A34097 人解整合素样金属蛋白酶10(ADAM10)ELISA试剂盒 Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit 96T/48T
A34098 人胸苷酸合成酶(TS)ELISA试剂盒 Human thymidylate synthetase,TS ELISA Kit 96T/48T
A34099 人胸腺嘧啶核苷磷酸化酶(TP)ELISA试剂盒 Human thymidinephosphorylase,TP ELISA Kit 96T/48T
A34100 人多聚ADP核糖聚合酶(PARP)ELISA试剂盒 Human Poly ADP ribose polymerase,PARP ELISA Kit 96T/48T
A34101 人葡萄糖激酶(GCK)ELISA试剂盒 Human glucokinase,GCK ELISA Kit 96T/48T
A34102 人单胺氧化酶(MAO)ELISA试剂盒 Human monoamine oxidase,MAO ELISA Kit 96T/48T
A34103 人胞浆型磷脂酶A2(cPLA2)ELISA试剂盒 Human cytosolic phospholipase A2,cPLA2 ELISA Kit 96T/48T
A34104 人二胺氧化酶(DAO)ELISA试剂盒 Human diamine oxidase,DAO ELISA Kit 96T/48T
A34105 人环加氧酶2(COX-2)ELISA试剂盒 Human cyclooxygenase-2,COX-2 ELISA Kit 96T/48T
A34106 人周期素依赖性激酶5(CDK5)ELISA试剂盒 Human Cyclin Dependent Kinase 5,CDK5 ELISA Kit 96T/48T
A34107 人激肽释放酶10(KLK 10)ELISA试剂盒 Human Kallikrein 10,KLK 10 ELISA Kit 96T/48T
A34108 人磷酯酶Cγ链(PLCγ1)ELISA试剂盒 Human Phospholipase Cγ1,PLCγ1 ELISA Kit 96T/48T
A34109 人神经氨酸酶(NA)ELISA试剂盒 Human neuraminidase,NA ELISA Kit 96T/48T
A34110 人磷酯酰肌醇特异性磷酯酶C(PIPLC)ELISA试剂盒 Human phosphoinositide-specific phospholipase C,PIPLC ELISA Kit 96T/48T
A34111 人酪氨酸激酶2(Tyk-2)ELISA试剂盒 Human tyrosine kinase2,Tyk-2 ELISA Kit 96T/48T
A34112 人白介素1β转换酶(ICE)ELISA试剂盒 Human Interleukin 1β converting enzyme,ICE ELISA Kit 96T/48T
A34113 人整合素连接激酶1(ILK-1)ELISA试剂盒 Human integrin-linked kinase 1,ILK-1 ELISA Kit 96T/48T
A34114 人细胞色素P450c21B/21-羟化酶(CYP21B)ELISA试剂盒 Human Cytochrome P450c21/21-hydroxylase,CYP21B ELISA Kit 96T/48T
A34115 人细胞色素P450c21A/21-羟化酶(CYP21A)ELISA试剂盒 Human Cytochrome P450c21/21-hydroxylase,CYP21A ELISA Kit 96T/48T
A34116 人末端脱氧核苷酸转移酶(TdT)ELISA试剂盒 Human terminal deoxynucleotidyl transferase,TdT ELISA Kit 96T/48T
A34117 人吲哚胺2,3-双加氧酶(IDO)ELISA试剂盒 Human indoleamine 2,3-dioxygenase,IDO ELISA Kit 96T/48T
A34118 人脾脏酪氨酸激酶(Syk)ELISA试剂盒 Human spleen tyrosine kinase,Syk ELISA Kit 96T/48T
A34119 人丝氨酸/苏氨酸蛋白磷酸酶(STK)ELISA试剂盒 Human serine/threonine protein kinase,STK ELISA Kit 96T/48T
A34120 人白介素受体相关激酶(IRAK)ELISA试剂盒 Human interleukin receptor associated kinase,IRAK ELISA Kit 96T/48T
A34121 人磷酸烯醇式丙酮酸羧激酶(PCK)ELISA试剂盒 Human phosphoenolpyruvate carboxykinase,PCK ELISA Kit 96T/48T
A34122 人胰蛋白酶(trypsin)ELISA试剂盒 Human trypsin ELISA Kit 96T/48T
A34123 人β位淀粉样前体蛋白裂解酶1(BACE1)ELISA试剂盒 Human β-site APP-Cleaving Enzyme 1,1BACE1 ELISA Kit 96T/48T
A34124 人蛋白二硫化物异构酶前体(PDI)ELISA试剂盒 Human Protein disulfide-isomerase precursor,PDI ELISA Kit 96T/48T
A34125 人蛋白二硫化物异构酶A3(PDIA3)ELISA试剂盒 Human protein disulfide-isomerase A3,PDIA3 ELISA Kit 96T/48T
A34126 人山梨醇脱氢酶(SDH)ELISA试剂盒 Human sorbitol dehydrogenase,SDH ELISA Kit 96T/48T
A34127 人肽基脯氨酰顺反异构酶(PPI)ELISA试剂盒 Human Peptidyl prolyl cis/trans isomerase,PPI ELISA Kit 96T/48T
A34128 人鸟氨酸氨基甲酰转移酶(OCT)ELISA试剂盒 Human ornithine carbamoyl transferase,OCT ELISA Kit 96T/48T
A34129 人内脏脂肪特异性丝氨酸蛋白酶抑制剂(vaspin)ELISA试剂盒 Human Visceral adipose-specific serine protease inhibitor,vaspin ELISA Kit 96T/48T
A34130 人蛋白磷酸酶(PP)ELISA试剂盒 Human Protein Phosphatase,PP ELISA Kit 96T/48T
A34131 人硫氧还蛋白还原酶(TrxR)ELISA试剂盒 Human thioredoxin reductase,TrxR ELISA Kit 96T/48T
A34132 人天青杀素(AZU)ELISA试剂盒 Human azurocidin,AZU ELISA Kit 96T/48T
A34133 人血管紧张肽酶A(ATA)ELISA试剂盒 Human Angiotensinase SKOV-3/DDP细胞, 卵巢癌顺铂耐药株A,ATA ELISA Kit 96T/48T
A34134 人胰淀粉酶(PAMY)ELISA试剂盒 Human Pancreatic Amylase,PAMY ELISA Kit 96T/48T
A34135 人α烯醇化酶(αENOL)ELISA试剂盒 Human α-enolase,αENOL ELISA Kit 96T/48T
A34136 人胶原酶I(Collagenase I)ELISA试剂盒 Human Collagenase I ELISA Kit 96T/48T
A34137 人磷酸化蛋白激酶C(P-PKC)ELISA试剂盒 Human phospho Protein Kinase C,P-PKC ELISA Kit 96T/48T
A34138 人酪氨酸羟化酶(TH)ELISA试剂盒 Human tyrosine hydroxylase,TH ELISA Kit 96T/48T
A34139 人多巴胺脱羧酶(DDC)ELISA试剂盒 Human dopamine decarboxylase,DDC ELISA Kit 96T/48T