SKOV-3/DDP细胞，卵巢癌顺铂耐药株供应

SKOV-3/DDP细胞， 卵巢癌顺铂耐药株
A34140 人非神经元性烯醇化酶（NNE）ELISA试剂盒 Human non-neuronal enolase,NNE ELISA Kit 96T/48T
A34141 人多巴胺-β羟化酶（DBH）ELISA试剂盒 Human dopamine-β-hydroxylase,DBH ELISA Kit 96T/48T
A34142 人胆碱乙酰化酶（CHAc）ELISA试剂盒 Human choline acetylase,CHAc ELISA Kit 96T/48T
A34143 人异柠檬酸脱氢酶（ICD）ELISA试剂盒 SKOV-3/DDP细胞， 卵巢癌顺铂耐药株Human isocitrate dehydrogenase,ICD ELISA Kit 96T/48T
A34144 人胰蛋白酶原Ⅱ（Try-Ⅱ）ELISA试剂盒 Human Trypsinogen-2,Try-ⅡELISA Kit 96T/48T
A34145 人腺苷脱氨酶（ADA）ELISA试剂盒 Human adenosine deaminase,ADA ELISA Kit 96T/48T
A34146 人天门冬氨酸氨基转移酶（AST）ELISA试剂盒 Human Aspartate aminotransferase,AST ELISA Kit 96T/48T
初代SKOV-3/DDP细胞， 卵巢癌顺铂耐药株消化培养法
1. 准备：取各种已消毒的培养用品置于净化台面，紫外线消毒20分钟。开始工作前先洗手、75%酒精擦拭手至肘部。
2. 布局：点燃酒精灯，安装吸管帽。
3. 处理组织：把组织块置于烧杯中，用Hanks液漂洗2~3次，去除血污；如怀疑组织可能污染，可先置于含有青链霉素的混合液中30~60分钟。
4. 剪切：用眼科剪把组织切成2~3毫米大小的块，以便于消化。加入比组织块总量多30~50倍的胰蛋白酶液，然后一并倒入三角烧瓶中，结扎瓶口或塞以胶塞。
5. 消化：或用恒温水浴，或置于37℃温箱消化均可，消化中每隔20分钟应摇动一次，如用电磁恒温搅拌器消化更好。消化时间依组织块的大小和组织的硬度而定。
6. 分离：在消化过程中见消化液发混浊时，可用吸管吸出少许消化液在镜下观察，如组织已分散成细胞团或单个细胞，立即终止消化，随即通过适宜不锈钢筛，滤掉尚未充分消化开的组织块。低速（500~1000转/分）离心消化液5分钟，吸出上清，加入适量含有血清的培养液。
7. 计数：用计数板计数，如细胞悬液细胞密度过大，再补加培养液调整后，分装入培养瓶中。对大多数细胞来说，pH要求在7.2~7.4范围，培养液呈微红色，如颜色偏黄，说明液体变酸，可用NaHCO3调整。
8. 培养：置于36.5℃温箱培养，如用CO2温箱培养，瓶口需用纱布棉塞或螺旋帽堵塞，纱布塞易生霉菌，每次换液时需要换新塞。
A34069 人3-羟基-3-甲基戊二酰辅酶A合酶1（HMGCS1）ELISA试剂盒 human 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1,HMGCS1 ELISA Kit 96T/48T
A34070 人嗜酸性白血球相关之RNA水解酵素家族成员12（EAR12）ELISA试剂盒 human eosinophil-associated ribonuclease A family member 12,EAR12 ELISA Kit 96T/48T
A34071 人嗜酸性白血球相关之RNA水解酵素家族成员3（EAR3）ELISA试剂盒 human eosinophil-associated ribonuclease A family member 3,EAR3 ELISA Kit 96T/48T
A34072 人嗜酸性白血球相关之RNA水解酵素家族成员2（EAR2）ELISA试剂盒 human eosinophil-associated ribonuclease A family member 2 ELISA Kit 96T/48T
A34073 人二氢嘧啶酶样3（DPYSL3）ELISA试剂盒 human dihydropyrimidinase-like 3,DPYSL3 ELISA Kit 96T/48T
A34074 人色氨酰tRNA合成酶（WARS）ELISA试剂盒 human tryptophanyl-tRNA synthetase,WARS ELISA Kit 96T/48T
A34075 人己糖激酶3（HK3）ELISA试剂盒 human hexokinase 3,HK3 ELISA Kit 96T/48T
A34076 人血小板型磷酸果糖激酶（PFKP）ELISA试剂盒 human phosphofructokinase-plaet,PFKP ELISA Kit 96T/48T
A34077 人尿苷二磷酸葡萄糖神经酰胺葡萄糖基转移酶（UGCG）ELISA试剂盒 human UDP-glucose ceramide glucosyltransferase ELISA Kit 96T/48T
A34078 人凋亡相关半胱氨酸肽酶4（Casp4）ELISA试剂盒 human caspase 4-apoptosis-related cysteine peptidase,Casp4 ELISA Kit 96T/48T
A34079 人吡哆醛/吡哆醇维生素B6激酶（PDXK）ELISA试剂盒 human caspase 4-apoptosis-related cysteine peptidase,Casp4 ELISA Kit 96T/48T
A34080 人二氢嘧啶酶样2（DPYSL2）ELISA试剂盒 human dihydropyrimidinase-like 2,DPYSL2 ELISA Kit 96T/48T
A34081 人血清/糖皮质激素调节激酶2（GSK2）ELISA试剂盒 human serum/glucocorticoid regulated kinase 2,GSK2 ISA KIT 96T/48T
A34082 人丝裂原活化蛋白激酶激酶6（MKK6）ELISA试剂盒 human mitogen activated protein kinase kinase 6,MKK6 ELISA Kit 96T/48T
A34083 人吡哆醛/吡哆醇维生素B6磷酸酶（PDXP）ELISA试剂盒 human pyridoxal/pyridoxine vitamin B6-phosphatase,PDXP ELISA Kit 96T/48T
A34084 人T细胞特异性鸟苷三磷酸酶（Tgtp）ELISA试剂盒 Human T-cell specific GTPase,Tgtp ELISA Kit 96T/48T
A34085 人法尼基二磷酸法尼基转移酶1（FDFT1）ELISA试剂盒 Human farnesyl-diphosphate farnesyltransferase 1,FDFT1 ELISA Kit 96T/48T
A34086 人D-乳酸脱氢酶（D-LDH） ELISA试剂盒 Human D-Lactate Dehydrogenase,D-LDH ELISA Kit 96T/48T
A34087 人α1抗胰蛋白酶（α1-AT）ELISA试剂盒 Human α1-Antitrypsin,α1-AT ELISA Kit 96T/48T
A34088 人激肽释放酶1（KLK 1）ELISA试剂盒 Human Kallikrein 1,KLK 1 ELISA Kit 96T/48T
A34089 人磷酸化乙酰辅酶A（PaCoA）ELISA试剂盒 Human Phosphorylated acetyl CoA,PaCoA ELISA Kit 96T/48T
A34090 人磷酸甘油酸变位酶2（PGAM2）ELISA试剂盒 Human phosphoglycerate mutase 2,PGAM2 ELISA Kit 96T/48T
A34091 人前列腺素D合成酶（PTGDS）ELISA试剂盒 Human Prostaglandin D synthase,PTGDS ELISA Kit 96T/48T
A34092 人肽酰脯氨酰异构酶（亲环蛋白）样1（PPIL1）ELISA试剂盒 Human peptidylprolyl isomerase（cyclophilin）-like 1,PPIL1 ELISA Kit 96T/48T
A34093 人促分裂素原活化蛋白激酶激活的蛋白激酶3（MAPKAPK3）ELISA试剂盒 Human mitogen-activated protein kinase-activated protein kinase 3,MAPKAPK3 ELISA Kit 96T/48T
A34094 人血管紧张素Ⅰ转化酶（ACEⅠ）ELISA试剂盒 Human Angiotensin Ⅰ Converting Enzyme,ACEⅠ ELISA Kit 96T/48T
A34095 人胱天蛋白酶12（Casp-12）ELISA试剂盒 Human Caspase 12,Casp-12 ELISA Kit 96T/48T
A34096 人蛋白激酶A（PKA）ELISA试剂盒 Human Protein kinase A,PKA ELISA Kit 96T/48T
A34097 人解整合素样金属蛋白酶10（ADAM10）ELISA试剂盒 Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit 96T/48T
A34098 人胸苷酸合成酶（TS）ELISA试剂盒 Human thymidylate synthetase,TS ELISA Kit 96T/48T
A34099 人胸腺嘧啶核苷磷酸化酶（TP）ELISA试剂盒 Human thymidinephosphorylase,TP ELISA Kit 96T/48T
A34100 人多聚ADP核糖聚合酶（PARP）ELISA试剂盒 Human Poly ADP ribose polymerase,PARP ELISA Kit 96T/48T
A34101 人葡萄糖激酶（GCK）ELISA试剂盒 Human glucokinase,GCK ELISA Kit 96T/48T
A34102 人单胺氧化酶（MAO）ELISA试剂盒 Human monoamine oxidase,MAO ELISA Kit 96T/48T
A34103 人胞浆型磷脂酶A2（cPLA2）ELISA试剂盒 Human cytosolic phospholipase A2,cPLA2 ELISA Kit 96T/48T
A34104 人二胺氧化酶（DAO）ELISA试剂盒 Human diamine oxidase,DAO ELISA Kit 96T/48T
A34105 人环加氧酶2（COX-2）ELISA试剂盒 Human cyclooxygenase-2,COX-2 ELISA Kit 96T/48T
A34106 人周期素依赖性激酶5（CDK5）ELISA试剂盒 Human Cyclin Dependent Kinase 5,CDK5 ELISA Kit 96T/48T
A34107 人激肽释放酶10（KLK 10）ELISA试剂盒 Human Kallikrein 10,KLK 10 ELISA Kit 96T/48T
A34108 人磷酯酶Cγ链（PLCγ1）ELISA试剂盒 Human Phospholipase Cγ1,PLCγ1 ELISA Kit 96T/48T
A34109 人神经氨酸酶（NA）ELISA试剂盒 Human neuraminidase,NA ELISA Kit 96T/48T
A34110 人磷酯酰肌醇特异性磷酯酶C（PIPLC）ELISA试剂盒 Human phosphoinositide-specific phospholipase C,PIPLC ELISA Kit 96T/48T
A34111 人酪氨酸激酶2（Tyk-2）ELISA试剂盒 Human tyrosine kinase2,Tyk-2 ELISA Kit 96T/48T
A34112 人白介素1β转换酶（ICE）ELISA试剂盒 Human Interleukin 1β converting enzyme,ICE ELISA Kit 96T/48T
A34113 人整合素连接激酶1（ILK-1）ELISA试剂盒 Human integrin-linked kinase 1,ILK-1 ELISA Kit 96T/48T
A34114 人细胞色素P450c21B/21-羟化酶（CYP21B）ELISA试剂盒 Human Cytochrome P450c21/21-hydroxylase,CYP21B ELISA Kit 96T/48T
A34115 人细胞色素P450c21A/21-羟化酶（CYP21A）ELISA试剂盒 Human Cytochrome P450c21/21-hydroxylase,CYP21A ELISA Kit 96T/48T
A34116 人末端脱氧核苷酸转移酶（TdT）ELISA试剂盒 Human terminal deoxynucleotidyl transferase,TdT ELISA Kit 96T/48T
A34117 人吲哚胺2,3-双加氧酶（IDO）ELISA试剂盒 Human indoleamine 2,3-dioxygenase,IDO ELISA Kit 96T/48T
A34118 人脾脏酪氨酸激酶（Syk）ELISA试剂盒 Human spleen tyrosine kinase,Syk ELISA Kit 96T/48T
A34119 人丝氨酸/苏氨酸蛋白磷酸酶（STK）ELISA试剂盒 Human serine/threonine protein kinase,STK ELISA Kit 96T/48T
A34120 人白介素受体相关激酶（IRAK）ELISA试剂盒 Human interleukin receptor associated kinase,IRAK ELISA Kit 96T/48T
A34121 人磷酸烯醇式丙酮酸羧激酶（PCK）ELISA试剂盒 Human phosphoenolpyruvate carboxykinase,PCK ELISA Kit 96T/48T
A34122 人胰蛋白酶（trypsin）ELISA试剂盒 Human trypsin ELISA Kit 96T/48T
A34123 人β位淀粉样前体蛋白裂解酶1（BACE1）ELISA试剂盒 Human β-site APP-Cleaving Enzyme 1，1BACE1 ELISA Kit 96T/48T
A34124 人蛋白二硫化物异构酶前体（PDI）ELISA试剂盒 Human Protein disulfide-isomerase precursor,PDI ELISA Kit 96T/48T
A34125 人蛋白二硫化物异构酶A3（PDIA3）ELISA试剂盒 Human protein disulfide-isomerase A3,PDIA3 ELISA Kit 96T/48T
A34126 人山梨醇脱氢酶（SDH）ELISA试剂盒 Human sorbitol dehydrogenase,SDH ELISA Kit 96T/48T
A34127 人肽基脯氨酰顺反异构酶（PPI）ELISA试剂盒 Human Peptidyl prolyl cis/trans isomerase,PPI ELISA Kit 96T/48T
A34128 人鸟氨酸氨基甲酰转移酶（OCT）ELISA试剂盒 Human ornithine carbamoyl transferase,OCT ELISA Kit 96T/48T
A34129 人内脏脂肪特异性丝氨酸蛋白酶抑制剂（vaspin）ELISA试剂盒 Human Visceral adipose-specific serine protease inhibitor,vaspin ELISA Kit 96T/48T
A34130 人蛋白磷酸酶（PP）ELISA试剂盒 Human Protein Phosphatase,PP ELISA Kit 96T/48T
A34131 人硫氧还蛋白还原酶（TrxR）ELISA试剂盒 Human thioredoxin reductase,TrxR ELISA Kit 96T/48T
A34132 人天青杀素（AZU）ELISA试剂盒 Human azurocidin,AZU ELISA Kit 96T/48T
A34133 人血管紧张肽酶A（ATA）ELISA试剂盒 Human Angiotensinase SKOV-3/DDP细胞， 卵巢癌顺铂耐药株A,ATA ELISA Kit 96T/48T
A34134 人胰淀粉酶（PAMY）ELISA试剂盒 Human Pancreatic Amylase,PAMY ELISA Kit 96T/48T
A34135 人α烯醇化酶（αENOL）ELISA试剂盒 Human α-enolase,αENOL ELISA Kit 96T/48T
A34136 人胶原酶I（Collagenase I）ELISA试剂盒 Human Collagenase I ELISA Kit 96T/48T
A34137 人磷酸化蛋白激酶C（P-PKC）ELISA试剂盒 Human phospho Protein Kinase C,P-PKC ELISA Kit 96T/48T
A34138 人酪氨酸羟化酶（TH）ELISA试剂盒 Human tyrosine hydroxylase,TH ELISA Kit 96T/48T
A34139 人多巴胺脱羧酶（DDC）ELISA试剂盒 Human dopamine decarboxylase,DDC ELISA Kit 96T/48T