产品详情
HCC-9204细胞, 肝癌细胞
A34607 人多聚血清蛋白(PHSA)ELISA试剂盒 Human Poly Serium Antigen,PHSA ELISA Kit 96T/48T
A34608 人胞浆免疫球蛋白(CIg)ELISA试剂盒 Human cytoplasmic immunoglobulin,CIg ELISA Kit 96T/48T
A34609 人纤维介素蛋白(fgl2)ELISA试剂盒 Human Fibrinogen like peptide 2,fgl2 ELISA Kit 96T/48T
A34610 人泛素蛋白(Ub)ELISA试剂盒 Human Ubiquitin,Ub ELISA Kit 96T/48T
A34611 人类粘蛋白(ORM)ELISA试剂盒 HCC-9204细胞, 肝癌细胞Human orosomucoid,ORM ELISA Kit 96T/48T
A34612 人弹性蛋白(ELN)ELISA试剂盒 Human Elastin,ELN ELISA Kit 96T/48T
A34613 人套膜蛋白(iNV)ELISA试剂盒 Human Involucrin,iNV ELISA Kit 96T/48T
A34614 人肌腱蛋白R(TN-R)ELISA试剂盒 Human tenascin-R,TN-R ELISA Kit 96T/48T
初代HCC-9204细胞, 肝癌细胞消化培养法
1. 准备:取各种已消毒的培养用品置于净化台面,紫外线消毒20分钟。开始工作前先洗手、75%酒精擦拭手至肘部。
2. 布局:点燃酒精灯,安装吸管帽。
3. 处理组织:把组织块置于烧杯中,用Hanks液漂洗2~3次,去除血污;如怀疑组织可能污染,可先置于含有青链霉素的混合液中30~60分钟。
4. 剪切:用眼科剪把组织切成2~3毫米大小的块,以便于消化。加入比组织块总量多30~50倍的胰蛋白酶液,然后一并倒入三角烧瓶中,结扎瓶口或塞以胶塞。
5. 消化:或用恒温水浴,或置于37℃温箱消化均可,消化中每隔20分钟应摇动一次,如用电磁恒温搅拌器消化更好。消化时间依组织块的大小和组织的硬度而定。
6. 分离:在消化过程中见消化液发混浊时,可用吸管吸出少许消化液在镜下观察,如组织已分散成细胞团或单个细胞,立即终止消化,随即通过适宜不锈钢筛,滤掉尚未充分消化开的组织块。低速(500~1000转/分)离心消化液5分钟,吸出上清,加入适量含有血清的培养液。
7. 计数:用计数板计数,如细胞悬液细胞密度过大,再补加培养液调整后,分装入培养瓶中。对大多数细胞来说,pH要求在7.2~7.4范围,培养液呈微红色,如颜色偏黄,说明液体变酸,可用NaHCO3调整。
8. 培养:置于36.5℃温箱培养,如用CO2温箱培养,瓶口需用纱布棉塞或螺旋帽堵塞,纱布塞易生霉菌,每次换液时需要换新塞。
A34529 人甲状腺结合球蛋白(TBG)ELISA试剂盒 Human Thyroxine-Binding Globulin Assay,TBG ELISA Kit 96T/48T
A34530 人晚期糖基化终末产物(AGEs)ELISA试剂盒 Human advanced glycation end products,AGEs ELISA Kit 96T/48T
A34531 人晚期糖基化终末产物受体(RAGE/AGER)ELISA试剂盒 Human Receptor for advanced glycation end products, RAGE/AGER ELISA Kit 96T/48T
A34532 人胰多肽(PP)ELISA试剂盒 Human Pancreatic Polypeptide,PP ELISA Kit 96T/48T
A34533 人胰岛素自身抗体(IAA)ELISA试剂盒 Human insulin autoantibodies,IAA ELISA Kit 96T/48T
A34534 人谷氨酸脱羧酶(GAD)ELISA试剂盒 Human glutamic acid decarboxylase,GAD ELISA Kit 96T/48T
A34535 人胸腺五肽(TP-5)ELISA试剂盒 Human Thymopentin,TP-5 ELISA Kit 96T/48T
A34536 人未磷酸化类胰岛素生长因子结合蛋白-1ELISA试剂盒 Human Non-Phosphorylated Insulin-like growth factor binding protein 1 ELISA Kit 96T/48T
A34537 人甲状旁腺激素样蛋白(PLP)ELISA试剂盒 Human parathyroid hormone-like protein,PLP ELISA Kit 96T/48T
A34538 人葡萄糖依赖性胰岛素释放多肽(GIP)ELISA试剂盒 Human Glucose dependent insulin releasing polypeptide,GIP ELISA Kit 96T/48T
A34539 人胰岛素原(PI)ELISA试剂盒 Human Proinsulin,PI ELISA Kit 96T/48T
A34540 人胰岛素受体β(ISR-β)ELISA试剂盒 Human Insulin Receptor β,ISR-β ELISA Kit 96T/48T
A34541 人糖化血红蛋白A1c(GHbA1c)ELISA试剂盒 Human Glycated hemoglobin A1c,GHbA1c ELISA Kit 96T/48T
A34542 人胰高血糖素样肽1(GLP-1)ELISA试剂盒 Human ghcagons-like pepfide 1,GLP-1 ELISA Kit 96T/48T
A34543 人甲状腺刺激免疫球蛋白(TSI)ELISA试剂盒 human thyroid stimulating immunoglobulin,TSI ELISA Kit 96T/48T
A34544 人甲状旁腺激素(PTH)ELISA试剂盒 human Parathyroid hormone,PTH ELISA Kit 96T/48T
A34545 人甲肟前列腺素D2(PGD2-MOX)ELISA试剂盒 human Prostaglandin D2-methoxime,PGD2-MOX ELISA Kit 96T/48T
A34546 人高敏甲状腺素(u-T4)ELISA试剂盒 human Ultrasensitivity Thyroxine,u-T4 ELISA Kit 96T/48T
A34547 人高灵敏度促甲状腺激素(U-TSH)ELISA试剂盒 human ultrasensitive thyroid-stimulating hormone,U-TSH ELISA Kit 96T/48T
A34548 人叠氮胸苷(AZT)ELISA试剂盒 human Azidothymidine,AZT ELISA Kit 96T/48T
A34549 人真核翻译起始因子3a(eIF3a)ELISA试剂盒 Human Eukaryotic translation initiation factor 3a,eIF3a ELISA Kit 96T/48T
A34550 人磷酸化细胞外信号调节激酶(pERK)ELISA试剂盒 Human phospho-extracellular signal-regulated kinase,pERK ELISA Kit 96T/48T
A34551 人受磷蛋白(PLN)ELISA试剂盒 Human phospholamban,PLN ELISA Kit 96T/48T
A34552 人肿瘤坏死因子相关弱凋亡诱导因子(TWEAK)ELISA试剂盒 Human tumour necrosis factor related weak inducer of apoptosis,TWEAK ELISA Kit 96T/48T
A34553 人IPO-38蛋白(IPO38)ELISA试剂盒 Human IPO-38 ELISA Kit 96T/48T
A34554 人切除修复交叉互补基因1(ERCC1)ELISA试剂盒 human excision repair cross-complementation group 1,ERCC1 ELISA Kit 96T/48T
A34555 人磷酸化核因子κB抑制蛋白α(pIKBα)ELISA试剂盒 human phospho-inhibitory subunit of NF-κBα,pIKBα ELISA Kit 96T/48T
A34556 人桩蛋白(Pax)ELISA试剂盒 Human paxillin,Pax ELISA Kit 96T/48T
A34557 人小乳腺表皮粘蛋白(SBEM)ELISA试剂盒 Human small breast epithelial mucin,SBEM ELISA Kit 96T/48T
A34558 人类似60S核糖体蛋白L21(LOC382344)ELISA试剂盒 human Mus musculus similar to 60S ribosomal protein L12,LOC382344 ELISA Kit 96T/48T
A34559 人类粘蛋白2(ORM2)ELISA试剂盒 human orosomucoid 2,ORM2 ELISA Kit 96T/48T
A34560 人MAX二聚化蛋白1(mxd1)ELISA试剂盒 human MAX dimerization protein 1,mxd1 ELISA Kit 96T/48T
A34561 人水闸蛋白14(CLDN14)ELISA试剂盒 human claudin 14,CLDN14 ELISA Kit 96T/48T
A34562 人可溶性半乳糖苷结合凝集素3(Lgals3)ELISA试剂盒 human lectin-galactose binding-soluble 3,Lgals3 ELISA Kit 96T/48T
A34563 人主要穹窿蛋白(MVP)ELISA试剂盒 human major vault protein,MVP ELISA Kit 96T/48T
A34564 人泛素蛋白D(UBD)ELISA试剂盒 human ubiquitin D,UBD ELISA Kit 96T/48T
A34565 人toll样受体2(TLR2)ELISA试剂盒 human toll-like receptor 2,TLR2 ELISA Kit 96T/48T
A34566 人青少年2型血色素沉着症/幼年型血色病相关蛋白2(HFE2)ELISA试剂盒 human hemochromatosis type 2/uvenile-uman homolog,HFE2 LISA Kit 96T/48T
A34567 人卷曲蛋白8(FZD8)ELISA试剂盒 human frizzled homolog 8,FZD8 ELISA Kit 96T/48T
A34568 人蜗牛同源物2(SNAI2)ELISA试剂盒 human snail homolog 2,SNAI2 ELISA Kit 96T/48T
A34569 人高尔基蛋白73(GP-73)ELISA试剂盒 Human golgi protein 73,GP-73 ELISA Kit 96T/48T
A34570 人TU3A蛋白(TU3A)ELISA试剂盒 Human TU3A Protein ELISA Kit 96T/48T
A34571 人电子转移黄素蛋白β肽(ETFB)ELISA试剂盒 Human electron-transfer-flavoprotein beta polypeptide,ETFB ELISA Kit 96T/48T
A34572 人皮肤蛋白(DPT)ELISA试剂盒 Human dermato-pontin,DPT ELISAHCC-9204细胞, 肝癌细胞 Kit 96T/48T
A34573 人可溶性蛋白185(sp185/HER-2)ELISA试剂盒 Human soluble protein 185,sp185/HER-2 ELISA Kit 96T/48T
A34574 人降解加速因子(DAF)ELISA试剂盒 Human decay-accelerating factor,DAF ELISA Kit 96T/48T
A34575 人T细胞活化连接蛋白(LAT)ELISA试剂盒 Human Linker for activation of T cell,LAT ELISA Kit 96T/48T
A34576 人SH2携带蛋白(SHC/SLP-76)ELISA试剂盒 Human SH2-containing protein,SHC/SLP-76 ELISA Kit 96T/48T
A34577 人B细胞连接蛋白(BLNK)ELISA试剂盒 Human B-Cell Linker Protein,BLNK ELISA Kit 96T/48T
A34578 人尿素酶相关蛋白C(UreC)ELISA试剂盒 Human urease related protein C,UreC ELISA Kit 96T/48T
A34579 人衔接蛋白酶活化因子1(APAF1)ELISA试剂盒 Human adaptor molecule apoptotic peptidase activating factor 1,APAF1 ELISA Kit 96T/48T
A34580 人锚蛋白B(Ank-B)ELISA试剂盒 Human ankyrin B,Ank-B ELISA Kit 96T/48T
A34581 人潜伏膜蛋白1(LMP-1)ELISA试剂盒 Human latent membrane protein-1,LMP-1 ELISA Kit 96T/48T
A34582 人重组激活基因2(RAG-2)ELISA试剂盒 Human recombination activating gene 2,RAG-2 ELISA Kit 96T/48T
A34583 人重组激活基因1(RAG-1)ELISA试剂盒 Human recombination activating gene 1,RAG-1 ELISA Kit 96T/48T
A34584 人NOD样受体(NLR)ELISA试剂盒 Human NOD-like receptor9,NLR ELISA Kit 96T/48T
A34585 人视黄酸诱导基因/RIG-1样受体(RLR)ELISA试剂盒 Human retinoic acid inducible gene/RIG-like receptor,RLR-9 ELISA Kit 96T/48T
A34586 人清道夫受体B(SRB/CD36)ELISA试剂盒 Human scavenger receptor B,SRB ELISA Kit 96T/48T
A34587 人杀菌肽B(CecB)ELISA试剂盒 Human cecropin B,CecB ELISA Kit 96T/48T
A34588 人抗菌肽(Att)ELISA试剂盒 Human Attacin,Att ELISA Kit 96T/48T
A34589 人颗粒溶素(GNLY)ELISA试剂盒 Human granulysin,GNLY ELISA Kit 96T/48T
A34590 人富组蛋白(HTN5)ELISA试剂盒 Human histatin 5,HTN5 ELISA Kit 96T/48T
A34591 人免疫球蛋白样转录体受体(ILTsR/LIR/CD85)ELISA试剂盒 Human Ig-like transcripts Receptor,ILTsR ELISA Kit 96T/48T
A34592 人丝?血球凝集素(FHA)ELISA试剂盒 Human filamentous hemagglutinin,FHA ELISA Kit 96T/48T
A34593 人细胞绒毛蛋白/埃兹蛋白(CVL)ELISA试剂盒 Human cytovillin/ezrin,CVL ELISA Kit 96T/48T
A34594 人嗜环蛋白/亲环素A(CyPA)ELISA试剂盒 Human cyclophilin A,CyPA ELISA Kit 96T/48T
A34595 人肌养蛋白(dystrophin)ELISA试剂盒 Human dystrophin ELISA Kit 96T/48T
A34596 人胶原凝集素(Collectin)ELISA试剂盒 Human Collectin ELISA Kit 96T/48T
A34597 人伴侣蛋白10(CPN10)ELISA试剂盒 Human chaperonin 10,CPN10 ELISA Kit 96T/48T
A34598 人纤调蛋白(FMOD)ELISA试剂盒 Human fibromodulin,FMOD ELISA Kit 96T/48T
A34599 人钙网蛋白(CRT)ELISA试剂盒 Human Calreticulin,CRT ELISA Kit 96T/48T
A34600 人网膜素(omentin)ELISA试剂盒 Human omentin ELISA Kit 96T/48T
A34601 人基质γ羧基谷氨酸蛋白(MGP)ELISA试剂盒 Human Matrix Gla Protein,MGP ELISA Kit 96T/48T
A34602 人基质裂解蛋白/基质溶素(MAT)ELISA试剂盒 Human matrilysin,MAT ELISA Kit 96T/48T
A34603 人溶酶体相关膜蛋白2(HLAMP-2)ELISA试剂盒 Human human lysosomal-associated membrane protein 2,HLAMP-2 ELISA Kit 96T/48T
A34604 人软骨糖蛋白39(HC gp-39)ELISA试剂盒 Human Cartilage glycoprotein 39,HC gp-39 ELISA Kit 96T/48T
A34605 人不透光相关蛋白(OPAs)ELISA试剂盒 Human Opacity-associated proteins,OPAs ELISA Kit 96T/48T
A34606 人鞭毛蛋白(flagellin)ELISA试剂盒 Human flagellin ELISA Kit 96T/48T