产品详情
LL-PK1细胞, 猪肾细胞
A33357 人α谷胱甘肽S转移酶(α-GST)ELISA试剂盒 Human α-glutathione S-transferases,α-GST ELISA Kit 96T/48T
A33358 人同型半胱氨酸(Hcy)ELISA试剂盒 Human Homocysteic acid,Hcy ELISA Kit 96T/48T
A33359 人游离脂肪酸(FFA)ELISA试剂盒 Human free fatty acids,FFA ELISA Kit 96T/48T
A33360 LL-PK1细胞, 猪肾细胞人骨粘连蛋白(ON)ELISA试剂盒 Human osteonectin,ON ELISA Kit 96T/48T
A33361 人叶酸(FA)ELISA试剂盒 Human Folic acid,FA ELISA Kit 96T/48T
A33362 人内毒素(ET)ELISA试剂盒 Human endotoxin,ET ELISA Kit 96T/48T
A33363 人环孢素A(CsA)ELISA试剂盒 Human Cyclosporine A,CsA ELISA Kit 96T/48T
A33364 人白三烯C4(LTC4)ELISA试剂盒 Human Leukotriene C4,LT-C4 ELISA Kit 96T/48T
传代细胞操作步骤:
1)吸除培养瓶内旧培养液。
2)向瓶内加入胰蛋白酶和EDTA混合液少许,以能覆满瓶底为限。
3)置温箱中2~5分钟,当发现细胞质回缩,LL-PK1细胞, 猪肾细胞间隙增大后,立即终止消化。
4)吸除消化液,向瓶内注入Hanks液数毫升,轻轻转动培养瓶,把残余消化液冲掉。注意加Hanks液冲洗细胞时,动作要轻,以免把已松动的细胞冲掉流失,如用胰蛋白酶液单独消化,吸除胰蛋白酶液后,可不用Hanks液冲洗,直接加入培养液。
5)用吸管吸取营养液轻轻反复吹打瓶壁细胞,使之从瓶壁脱离形成细胞悬液。
6)计数板计数后,把细胞悬液分成等份分装入数个培养瓶中,置温箱中培养。
A33315 人甲状腺素(T4)ELISA试剂盒 Human thyroxine,T4 ELISA Kit 96T/48T
A33316 人游离甲状腺素(FT4)ELISA试剂盒 Human Free Thyroxine,FT4 ELISA Kit 96T/48T
A33317 人胰岛素(INS)ELISA试剂盒 Human Insulin,INS ELISA Kit 96T/48T
A33318 人C肽(C-Peptide)ELISA试剂盒 Human C-Peptide ELISA Kit 96T/48T
A33319 人绒毛膜促性腺激素(HCG)ELISA试剂盒 Human Chorionic Gonadotrophin,HCG ELISA Kit 96T/48T
A33320 人促黄体激素(LH)ELISA试剂盒 Human luteotropic hormone,LH ELISA Kit 96T/48T
A33321 人瘦素(LEP)ELISA试剂盒 Human Leptin,LEP ELISA Kit 96T/48T
A33322 人生长激素(HGH)ELISA试剂盒 Human growth hormone,HGH ELISA Kit 96T/48T
A33323 人红细胞生成素(EPO)ELISA试剂盒 Human Erythropoietin,EPO ELISA Kit 96T/48T
A33324 人前列腺素F2α(PGF2α)ELISA试剂盒 Human Prostaglandin F2α,PGF2α ELISA Kit 96T/48T
A33325 人抗人类免疫缺陷病毒抗体(HIV)ELISA试剂盒 Human anti-immunodeficiency virus antibody,HIV ELISA Kit 96T/48T
A33326 人载脂蛋白C3(Apo C3)ELISA试剂盒 Human Apolipoprotein C3,Apo C3 ELISA Kit 96T/48T
A33327 人胆固醇(CH)ELISA试剂盒 Human cholesterol,CH ELISA Kit 96T/48T
A33328 人金属硫蛋白2(MT2)ELISA试剂盒 Human Metallothionein 2,MT2 ELISA Kit 96T/48T
A33329 人3-硝基酪氨酸(3-NT)ELISA试剂盒 Human 3-Nitrotyrosine,3-NT ELISA Kit 96T/48T
A33330 人糖胺聚糖(GAG)ELISA试剂盒 Human glycosaminoglycan,GAG ELISA Kit 96T/48T
A33331 人半胱氨酰白三烯(CysLTs)ELISA试剂盒 Human cysteinyl leukotrienes,CysLTs ELISA Kit 96T/48T
A33332 人钙腔蛋白(CALU)ELISA试剂盒 Human calumenin,CALU ELISA Kit 96T/48T
A33333 人载脂蛋白A5(apo-A5)ELISA试剂盒 Human apoprotein A5,apo-A5 ELISA Kit 96T/48T
A33334 人视黄醇结合蛋白(RBP)ELISA试剂盒 Human Retinol binding protein,RBP ELISA Kit 96T/48T
A33335 人8羟基脱氧鸟苷(8-OHdG)ELISA试剂盒 Human hydroxylysine,Hyl ELISA Kit 96T/48T
A33336 人载脂蛋白H(Apo-H)ELISA试剂盒 Human Apolipoprotein H,apo-H ELISA Kit 96T/48T
A33337 人美洲商陆素(PWM)ELISA试剂盒 Human pokeweed mitogen,PWM ELISA Kit 96T/48T
A33338 人脂多糖/内毒素(LPS)ELISA试剂盒 Human Lipopolysaccharides,LPS ELISA Kit 96T/48T
A33339 人preptin ELISA试剂盒 Human preptin ELISA Kit 96T/48T
A33340 人载脂蛋白E(Apo-E)ELISA试剂盒 Human Apolipoprotein E,Apo-E ELISA Kit 96T/48T
A33341 人成骨生长肽(OGP)ELISA试剂盒 Human Osteogenic Growth Peptide,OGP ELISA Kit 96T/48T
A33342 人谷胱甘肽(GSH)ELISA试剂盒 Human glutathione,GSH ELISA Kit 96T/48T
A33343 人17-酮类固醇(17-KS)ELISA试剂盒 Human 17-ketosteroids,17-KS ELISA Kit 96T/48T
A33344 人17羟皮质类固醇(17-OHCS)ELISA试剂盒 Human 17-Hydroxycorticosteroids,17-OHCS ELISA Kit 96T/48T
A33345 人组织蛋白酶K(cath-K)ELISA试剂盒 Human Cathepsin K,cath-K ELISA Kit 96T/48T
A33346 人骨形成蛋白(BMPs)ELISA试剂盒 Human bone morphogenetic proteins,BMPs ELISA Kit 96T/48T
A33347 人血凝素(HA)ELISA试剂盒 Human hemagglutinin,HA ELISA Kit 96T/48T
A33348 人脂肪分化相关蛋白(ADRP)ELISA试剂盒 Human adipose differentiation-related protein,ADRP ELISA Kit 96T/48T
A33349 人钙调结合蛋白(CALD)ELISA试剂盒 Human Caldesmon,CALD ELISA Kit 96T/48T
A33350 人钙调磷酸酶(CaN)ELISA试剂盒 Human calcineurin,CaN ELISA Kit 96T/48T
A33351 人低密度脂蛋白(LDL)ELISA试剂盒 Human low density lipoprotein,LDL ELISA Kit 96T/48T
A33352 人骨碱性磷酸酶(BALP)ELISA试剂盒 Human bone alkalineLL-PK1细胞, 猪肾细胞 phosphatase,BALP ELISA Kit 96T/48T
A33353 人环磷酰胺(CTX)ELISA试剂盒 Human Cyclophosphamide,CTX ELISA Kit 96T/48T
A33354 人β羟丁酸(β-OHB)ELISA试剂盒 Human Beta-Hydroxybutyric Acid,β-OHB ELISA Kit 96T/48T
A33355 人高密度脂蛋白胆固醇(HDL-C)ELISA试剂盒 Human High density lipoprotein cholesterol,HDL-C ELISA Kit 96T/48T
A33356 人高密度脂蛋白(HDL)ELISA试剂盒 Human High density lipoprotein,HDL ELISA Kit 96T/48T