产品详情
Sf-900™ III SFM (1X),liquid ,英文名: Sf-900™ III SFM (1X), liquid ,规格: 500ml ,货号: 12658-019 ,品牌 GIBCO
Sf-900™ III SFM is a low-hydrolysate, protein-free, animal origin-free medium that provides consistency, and reliability in cell growth. Because this medium does not include animal-derived components, the need to screen for adventitious agents is reduced.
Optimized to support and maximize Sf9 and Sf21 cell growth and recombinant gene expression in suspension culture using both baculovirus and stable insect expression systems Supports Sf9 cell growth to densities of 10 to 14 x 106 cells/ml Protein-free, low-hydrolysate formulation improves lot-to-lot consistency and reliability Contains no components of animal origin,facilitating the regulatory process
Storage:Store in the dark at 2 to 8°C.
Gibco Sf-900 III SFM (1X),liquid详细描述:
Sf-900™ III SFM (1X),liquidForm: Liquid
Brand: Sf-900™, GIBCO®
Species: Spodoptera frugiperda
Cell Line: Sf9, Sf21
Glutamine: L-Glutamine
Serum Level: Serum-Free
Product Size: 500 ml
Classification: Protein-Free, Animal Origin-Free, Serum-Free
更多GIBCO血清产品
保存血清的方法:Sf-900™ III SFM (1X),liquid应保存在-5度至-20度。然而,若存放于4度时,请勿超过一个月。若您一次无法用完一瓶,建议将无菌分装血清至恰当的灭菌容器内,再放回冷冻。
人白色念珠菌(C.albicans)ELISA试剂盒 M00200 Human Candida Albicans,C.albicans ELISA Kit 96T/48T
人流行性乙型脑炎抗体IgG(JE IgG)ELISA试剂盒 M00201 Human Coronaviruses IgG ELISA Kit 96T/48T
人沙眼衣原体抗体(CT)ELISA试剂盒 M00202 Human Chlamydia trachomatis,CT ELISA Kit 96T/48T
人血吸虫(schistosoma)ELISA试剂盒 M00203 human schistosoma ELISA Kit 96T/48T
人轮状病毒抗原(RV Ag)ELISA试剂盒 M00204 Human Rotavirus antigen,RV Ag ELISA Kit 96T/48T
人军团菌抗原(LP Ag)ELISA试剂盒 M00205 Human Legionella pneumophila antigen,LP Ag ELISA Kit 96T/48T
人大肠菌素(colicin)ELISA试剂盒 M00206 human colicin ELISA Kit 96T/48T
人腺病毒抗原(ADV-Ag)ELISA试剂盒 M00207 Human Adenovirus Antigen,ADV-Ag ELISA Kit 96T/48T
人旋毛虫抗体(Trichinella Ab)ELISA试剂盒 M00208 Human Trichinella antibody ELISA Kit 96T/48T
人破伤风抗体(Tetanus Ab)ELISA试剂盒 M00209 Human Tetanus Antibody ELISA Kit 96T/48T
人钩端螺旋体IgM(Lebtospira)ELISA试剂盒 M00210 Human Lebtospira IgM ELISA Kit 96T/48T
人钩端螺旋体IgG(Lep IgG)ELISA试剂盒 M00211 Human Leptospira IgG,Lep IgG ELISA Kit 96T/48T
人布鲁氏菌抗体IgG(Brucella Ab IgG)ELISA试剂盒 M00212 Human Brucella Antibody IgG,Brucella Ab IgG ELISA Kit 96T/48T
人水痘带状疱疹病毒IgM(VZV-IgM)ELISA试剂盒 M00213 Human Varicella zoster virus IgM,VZV IgM ELISA Kit 96T/48T
人水痘带状疱疹病毒IgG(VZV-IgG)ELISA试剂盒 M00214 Human Varicella zoster virus IgG,VZV-IgG ELISA Kit 96T/48T
人军团菌抗体IgA(LP Ab-IgA)ELISA试剂盒 M00215 Human Legionella antibody IgA,LP Ab-IgA ELISA Kit 96T/48T
人军团菌抗体IgM(LP Ab-IgM )ELISA试剂盒 M00216 Human Legionella antibody IgM,LP Ab-IgM ELISA Kit 96T/48T
人军团菌抗体IgG(LP Ab-IgG)ELISA试剂盒 M00217 Human Legionella antibody IgG,LP Ab-IgG ELISA Kit 96T/48T
人腮腺炎病毒IgM ELISA试剂盒 M00218 Human paramyxovirus parotitis IgM ELISA Kit 96T/48T
人腮腺炎病毒IgG ELISA试剂盒 M00219 Human paramyxovirus parotitis IgG ELISA Kit 96T/48T
人EB病毒IgM(EBv IgM)ELISA试剂盒 M00220 Human epstein-barr virus IgM,EBv IgM ELISA Kit 96T/48T
人EB病毒IgA(EBv IgA)ELISA试剂盒 M00221 Human epstein-barr virus IgA,EBv IgA ELISA Kit 96T/48T
人EB病毒IgG(EBv IgG)ELISA试剂盒 M00222 Human epstein-barr virus IgG,EBv IgG ELISA Kit 96T/48T
人登革热抗体(DF-Ab)ELISA试剂盒 M00223 Human dengue fever antibody,DF ELISA Kit 96T/48T
人流感病毒抗体IgG(FLU)ELISA试剂盒 M00224 Human Influenza viruses IgG,FLU ELISA Kit 96T/48T
人腺病毒IgM(ADV-IgM)ELISA试剂盒 M00225 Human Adenovirus IgM,ADV-IgM ELISA Kit 96T/48T
人腺病毒IgG(ADV-IgG)ELISA试剂盒 M00226 Human Adenovirus IgG,ADV-IgG ELISA Kit 96T/48T
人轮状病毒(RV)IgM ELISA试剂盒 M00227 Human Rotavirus,RV IgM ELISA Kit 96T/48T
人艾柯病毒IgM(ECHO IgM)ELISA试剂盒 M00228 human EchoVirus IgM,ECHO IgM ELISA Kit 96T/48T
人抗呼吸道合胞病毒抗体(RSV)ELISA试剂盒 M00229 Human anti-respiratory syncytial virus antibody,RSV ELISA Kit 96T/48T
人麻疹病毒IgM(MV IgM)ELISA试剂盒 M00230 Human measlesvirus IgM,MV IgM ELISA Kit 96T/48T
人柯萨奇病毒IgM(Cox V-IgM)ELISA试剂盒 M00231 Human Coxsackie virus IgM,Cox V-IgM ELISA Kit 96T/48T
人柯萨奇病毒IgG(Cox V-IgG)ELISA试剂盒 M00232 Human Coxsackie virus IgG,Cox V-IgG ELISA Kit 96T/48T
人结核菌杆抗体IgG(TB-Ab IgG)ELISA试剂盒 M00233 Human tuberculosis antibody IgG,TB-Ab IgG ELISA Kit 96T/48T
人庚型肝炎病毒IgM(HGV-IgM)ELISA试剂盒 M00234 Human Hepatitis G virus IgG,HGV-IgG ELISA Kit 96T/48T
人戊型肝炎病毒IgM(HEV-IgM)ELISA试剂盒 M00235 Human Hepatitis D virus IgM,HDV IgM ELISA KitSf-900™ III SFM (1X),liquid 96T/48T
人戊型肝炎病毒IgG(HEV-IgG)ELISA试剂盒 M00236 human hepatitis E virus IgG,HEV-IgG ELISA Kit 96T/48T
人丁型肝炎IgM(HDV IgM)ELISA试剂盒 M00237 Human Hepatitis D virus IgM,HDV IgM ELISA Kit 96T/48T
如何解冻血清才不会使产品质量受损?将血清从冷冻箱取出后,先置于2-8度冰箱使之溶解,然后在室温下使之全溶。但必须注意的是,溶解过程中必须规则地摇晃均匀。
何谓热灭活?一般是以56度,30分钟来处理已解冻的血清,因为此加热步骤,可以使补体去活化,而补体所参与的反应有:溶细胞活性,平滑肌的收缩,肥大细胞和血小板组胺的释放,增强吞噬作用,淋巴细胞、巨噬细胞的趋化和激活。
有必要做热灭活吗?实验显示,经过正确处理的热灭活血清,对大多数的细胞而言是不需要的。经此处理过的血清对细胞的生长只有微小的促进,或*没有任何作用,甚至通常因为高温处理影响了血清的质量,而造成细胞生长速率的降低。而经过热处理的血清,沉淀物的形成会显著的增多,这些沉淀物在倒立显微镜下观察,像是“小黑点”,常常会让研究者误以为是血清遭受污染,而把血清放在37度环境中,又会使此沉淀物更增多,使研究者误认为是微生物的分裂扩增。
因此我们建议大家,Sf-900™ III SFM (1X),liquid热灭活若非必须,您可以不需要做热处理这一步。如此一来,不但节省您宝贵的时间,更确保您血清的质量!另外,购买已灭活成品也不失一个捷径,请在购买的时候注意询问厂家是否是已灭活血清。