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人乳腺癌*耐药株,MCF7 ADR细胞
MCF7 ADR 贴壁上皮细胞
(2)客户自备试剂
1)PBS
2)RPMI-1640培养基(不含Hepes)+ 10%胎牛血清+ 1%双抗
3)0.25% (W/V)*(含0.02% EDTA)
人乳腺癌*耐药株,MCF7 ADR细胞 (3)细胞背景
1)生长方式:贴壁
2)种属:Homo sapiens 人
(4)培养条件
1)培养基:RPMI-1640培养基(不含Hepes)+ 10%胎牛血清+ 1%双抗
2)温度:37.0°C
3)气体:空气 95%,CO2 5%
(5)培养方法
收到细胞后,在倒置显微镜下观察整个细胞生长情况:
如果细胞未长满,用75%酒精喷洒整个瓶消毒后放到超菌台内,严格无菌操作,打开细胞培养瓶,留10ml培养液继续培养。
如果细胞已长满(达80-90%)。即可进行传代,具体步骤如下:
1)弃去培养液,用PBS洗1-2次。
2)向瓶内加入1.0-2.0ml*液,在倒置显微镜下观察细胞消化情况,若细胞大部分变圆,迅速拿回操作台,吸取*,加含有6ml 含10%血清的培养液,轻轻吹打细胞。
3)加入等量的的培养液,轻轻吹打混匀后吸出一半,分到新的培养
4)传代比例:1:2-1:3
人乳腺癌*耐药株,MCF7 ADR细胞(6)常见问题及解决方案
1)培养瓶有破裂,培养液有漏液:细胞*可能会污染,所以我们会及时安排帮老师解决。
2)细胞漂浮:培养瓶不开封,瓶口酒精擦拭后平躺放置在培养箱。次日观察,如细胞大部分又贴回瓶底,表明细胞活力正常,剩余漂浮的细胞可以去掉,留10ml培养液培养观察,细胞生长至汇合度80%,进行消化传代;如细胞还是不贴壁,将细胞离心收集转到新培养瓶,原培养瓶加部分培养液继续培养,中间注意观察,我们的技术人员会一直跟踪指导,直到问题解决。
更多细胞产品!
细胞分离、培养、鉴定:
(1)从中国正常人、中国病人、小鼠、大鼠、兔、猪、牛等等组织中对原代细胞进行分离、培养、鉴定:我们将以客户要求为标准,提供多种属如中国正常人、中国病人、小鼠、大鼠、兔、猪、牛、其它动物原代细胞的分离、培养、鉴定,并提供相应的文件、资料、数据和检测证书。对于人源性细胞,必要时,在许可的条件下,我们尽可能的满足客户所提供的要求。
(2)从建立实验性疾病动物(小鼠、大鼠、兔)模型体内组织中对人乳腺癌*耐药株,MCF7 ADR细胞进行分离、培养、鉴定: 我们将以客户要求,建立标准实验性疾病动物模型,或客户提供实验性疾病动物模型,对原代细胞进行分离、培养、鉴定,并提供相应的文件、资料、数据和检测证书。13708 Janus激酶2(JAK2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Janus Kinase 2 (JAK2) 48T/96T Rattus norvegicus (Rat)
13709 旁血小板溶蛋白(PPL)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Periplakin (PPL) 48T/96T Homo sapiens (Human)
13710 Engrailed同源框蛋白2(EN2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Engrailed Homeobox Protein 2 (EN2) 48T/96T Homo sapiens (Human)
13711 胶质细胞系来源神经营养因子受体α1(GFRα1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1) 48T/96T Homo sapiens (Human)
13712 肿瘤坏死因子α诱导蛋白6(TNFαIP6)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6) 48T/96T Mus musculus (Mouse)
13713 多聚A特异性核糖核酸酶(PARN)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Poly A Specific Ribonuclease (PARN) 48T/96T Homo sapiens (Human)
13714 氯化物细胞内通道蛋白1(CLIC1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Chloride Intracellular Channel Protein 1 (CLIC1) 48T/96T Homo sapiens (Human)
13715 醌型二氢蝶啶还原酶(QDPR)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Quinoid Dihydropteridine Reductase (QDPR) 48T/96T Homo sapiens (Human)
13716 肌酸转运蛋白(CRT)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Creatine Transporter (CRT) 48T/96T Homo sapiens (Human)
13717 蛋氨酸腺苷转移酶Ⅰα(MAT1α)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Methionine Adenosyltransferase I Alpha (MAT1a) 48T/96T Mus musculus (Mouse)
13718 双氧化酶2(DUOX2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Dual Oxidase 2 (DUOX2) 48T/96T Homo sapiens (Human)
13719 双氧化酶2(DUOX2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Dual Oxidase 2 (DUOX2) 48T/96T Rattus norvegicus (Rat)
13720 组蛋白H1(H1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Histone H1 (H1) 48T/96T Homo sapiens (Human)
13721 苯*羟化酶(PAH)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Phenylalanine Hydroxylase (PAH) 48T/96T Homo sapiens (Human)
13722 苏氨酰tRNA合成酶(TARS)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Threonyl tRNA Synthetase (TARS) 48T/96T Homo sapiens (Human)
13723 γ-谷氨酰水解酶(γGH)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Gamma-Glutamyl Hydrolase (gGH) 48T/96T Homo sapiens (Human)
13724 胃泌酸调节素(OXM)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Oxyntomodulin (OXM) 48T/96T Homo sapiens (Human)
13725 硒结合蛋白1(SELENBP1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Selenium Binding Protein 1 (SELENBP1) 48T/96T Homo sapiens (Human)
13726 细胞色素P450家族成员2E1(CYP2E1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cytochrome P450 2E1 (CYP2E1) 48T/96T Mus musculus (Mouse)
13727 衰老关键蛋白2(FBLN2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibulin 2 (FBLN2) 48T/96T Homo sapiens (Human)
13728 单核细胞趋化蛋白1(MCP1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1) 48T/96T Sus scrofa; Porcine (Pig)
13729 趋化因子C-X-C-基元受体5(CXCR5)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Chemokine C-X-C-Motif Receptor 5 (CXCR5) 48T/96T Homo sapiens (Human)
13730 白介素13受体α2(IL13Rα2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interleukin 13 Receptor Alpha 2 (IL13Ra2) 48T/96T Mus musculus (Mouse)
13731 层黏连蛋白α3(LAMα3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Laminin Alpha 3 (LAMa3) 48T/96T Homo sapiens (Human)
13732 *丰富重复激酶2(LRRK2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Leucine Rich Repeat Kinase 2 (LRRK2) 48T/96T Homo sapiens (Human)
13733 甘露糖受体C2(MRC2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Mannose Receptor C Type 2 (MRC2) 48T/96T Homo sapiens (Human)
13734 E1A激活基因阻遏子1(CREG1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cellular Repressor Of E1A Stimulated Genes 1 (CREG1) 48T/96T Homo sapiens (Human)
13735 E1A激活基因阻遏子1(CREG1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cellular Repressor Of E1A Stimulated Genes 1 (CREG1) 48T/96T Mus musculus (Mouse)
13736 维甲酸受体α(RARα)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Retinoic Acid Receptor Alpha (RARa) 48T/96T Rattus norvegicus (Rat)
13737 白介素33(IL33)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interleukin 33 (IL33) 48T/96T Rattus norvegicus (Rat)
13738 白细胞衍生趋化因子1(LECT1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Leukocyte Cell Derived Chemotaxin 1 (LECT1) 48T/96T Homo sapiens (Human)
13739 轻肽铁蛋白(FTL)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Ferritin, Light Polypeptide (FTL) 48T/96T Rattus norvegicus (Rat)
13740 沉默调节蛋白6(SIRT6)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Sirtuin 6 (SIRT6) 48T/96T Homo sapiens (Human)
13741 连环蛋白β1(CTNNβ1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Catenin Beta 1 (CTNNb1) 48T/96T Rattus norvegicus (Rat)
13742 钙蛋白酶1(CAPN1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Calpain 1, Large Subunit (CAPN1) 48T/96T Homo sapiens (Human)
13743 糖原合酶激酶3β(GSK3β)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Glycogen Synthase Kinase 3 Beta (GSK3b) 48T/96T Rattus norvegicus (Rat)
13744 线粒体外膜转位酶70A(TOMM70A)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A) 48T/96T Homo sapiens (Human)
13745 转甲状腺素蛋白(TTR)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Transthyretin (TTR) 48T/96T Sus scrofa; Porcine (Pig)
13746 分拣连接蛋白13(SNX13)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Sorting Nexin 13 (SNX13) 48T/96T Homo sapiens (Human)
13747 分拣连接蛋白17(SNX17)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Sorting Nexin 17 (SNX17) 48T/96T Homo sapiens (Human)
13748 微纤丝关联蛋白4(MFAP4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Microfibrillar Associated Protein 4 (MFAP4) 48T/96T Homo sapiens (Human)
13749 脑多巴胺神经营养因子(CDNF)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cerebral Dopamine Neurotrophic Factor (CDNF) 48T/96T Rattus norvegicus (Rat)
13750 髓细胞触发受体2(TREM2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) 48T/96T Mus musculus (Mouse)
13751 细胞程序性死亡蛋白6(PDCD6)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Programmed Cell Death Protein 6 (PDCD6) 48T/96T Homo sapiens (Human)
13752 细胞程序性死亡蛋白6相互作用蛋白(PDCD6IP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP) 48T/96T Homo sapiens (Human)
13753 纤调蛋白(FMOD)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibromodulin (FMOD) 48T/96T Rattus norvegicus (Rat)
13754 Ⅴ型胶原α2(COL5α2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Collagen Type V Alpha 2 (COL5a2) 48T/96T Mus musculus (Mouse)
13755 细胞色素P450家族成员1A2(CYP1A2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cytochrome P450 1A2 (CYP1A2) 48T/96T Homo sapiens (Human)
13756 C1q肿瘤坏死因子相关蛋白9(C1QTNF9)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) 48T/96T Homo sapiens (Human)
13757 角蛋白16(KRT16)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Keratin 16 (KRT16) 48T/96T Homo sapiens (Human)
13758 层粘连蛋白γ1(LAMγ1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Laminin Gamma 1 (LAMC1) 48T/96T Homo sapiens (Human)
13759 Smad同源物2(Smad2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Mothers Against Decapentaplegic Homolog 2 (Smad2) 48T/96T Mus musculus (Mouse)
13760 神经外营养蛋白1(NXPH1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Neurexophilin 1 (NXPH1) 48T/96T Homo sapiens (Human)
13761 唾液酸酶2(SIAL2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Sialidase 2, Cytosolic (SIAL2) 48T/96T Rattus norvegicus (Rat)
13762 肽基*脱亚氨酶Ⅵ(PADI6)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Peptidyl Arginine Deiminase Type VI (PADI6) 48T/96T Homo sapiens (Human)
13763 原肌球调节蛋白3(TMOD3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tropomodulin 3 (TMOD3) 48T/96T Mus musculus (Mouse)
13764 弹性蛋白微原纤维界面因子2(EMILIN2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Elastin Microfibril Interface Located Protein 2 (EMILIN2) 48T/96T Mus musculus (Mouse)
13765 白介素12受体β2(IL2Rβ2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interleukin 12 Receptor Beta 2 (IL12Rb2) 48T/96T Homo sapiens (Human)
13766 组胺H3受体(HRH3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Histamine Receptor H3 (HRH3) 48T/96T 人乳腺癌*耐药株,MCF7 ADR细胞 Rattus norvegicus (Rat)
13767 角蛋白81(KRT81)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Keratin 81 (KRT81) 48T/96T Homo sapiens (Human)