产品详情
人乳腺癌*耐药株,MCF ADR细胞
MCF ADR 贴壁上皮细胞
(2)客户自备试剂
1)PBS
2)RPMI-1640培养基(不含Hepes)+ 10%胎牛血清+ 1%双抗
3)0.25% (W/V)*(含0.02% EDTA)
人乳腺癌*耐药株,MCF ADR细胞(3)细胞背景
1)生长方式:贴壁
2)种属:Homo sapiens 人
(4)培养条件
1)培养基:RPMI-1640培养基(不含Hepes)+ 10%胎牛血清+ 1%双抗
2)温度:37.0°C
3)气体:空气 95%,CO2 5%
(5)培养方法
收到细胞后,在倒置显微镜下观察整个细胞生长情况:
如果细胞未长满,用75%酒精喷洒整个瓶消毒后放到超菌台内,严格无菌操作,打开细胞培养瓶,留10ml培养液继续培养。
如果细胞已长满(达80-90%)。即可进行传代,具体步骤如下:
1)弃去培养液,用PBS洗1-2次。
2)向瓶内加入1.0-2.0ml*液,在倒置显微镜下观察细胞消化情况,若细胞大部分变圆,迅速拿回操作台,吸取*,加含有6ml 含10%血清的培养液,轻轻吹打细胞。
3)加入等量的的培养液,轻轻吹打混匀后吸出一半,分到新的培养
4)传代比例:1:2-1:3
人乳腺癌*耐药株,MCF ADR细胞(6)常见问题及解决方案
1)培养瓶有破裂,培养液有漏液:细胞*可能会污染,所以我们会及时安排帮老师解决。
2)细胞漂浮:培养瓶不开封,瓶口酒精擦拭后平躺放置在培养箱。次日观察,如细胞大部分又贴回瓶底,表明细胞活力正常,剩余漂浮的细胞可以去掉,留10ml培养液培养观察,细胞生长至汇合度80%,进行消化传代;如细胞还是不贴壁,将细胞离心收集转到新培养瓶,原培养瓶加部分培养液继续培养,中间注意观察,我们的技术人员会一直跟踪指导,直到问题解决。
更多细胞产品!
细胞分离、培养、鉴定:
(1)从中国正常人、中国病人、小鼠、大鼠、兔、猪、牛等等组织中对原代细胞进行分离、培养、鉴定:我们将以客户要求为标准,提供多种属如中国正常人、中国病人、小鼠、大鼠、兔、猪、牛、其它动物原代细胞的分离、培养、鉴定,并提供相应的文件、资料、数据和检测证书。对于人源性细胞,必要时,在许可的条件下,我们尽可能的满足客户所提供的要求。
(2)从建立实验性疾病动物(小鼠、大鼠、兔)模型体内组织中对人乳腺癌*耐药株,MCF ADR细胞进行分离、培养、鉴定: 我们将以客户要求,建立标准实验性疾病动物模型,或客户提供实验性疾病动物模型,对原代细胞进行分离、培养、鉴定,并提供相应的文件、资料、数据和检测证书。
13768 分泌球蛋白家族2A成员2(SCGB2A2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Secretoglobin Family 2A, Member 2 (SCGB2A2) 48T/96T Homo sapiens (Human)
13769 肌球蛋白轻链9(MYL9)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Myosin Light Chain 9, Regulatory (MYL9) 48T/96T Homo sapiens (Human)
13770 半*丰富跨膜BMP调节因子1(CRIM1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1) 48T/96T Homo sapiens (Human)
13771 几丁质酶3样蛋白2(CHI3L2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Chitinase 3 Like Protein 2 (CHI3L2) 48T/96T Homo sapiens (Human)
13772 *结合蛋白3(RBP3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Retinol Binding Protein 3, Interstitial (RBP3) 48T/96T Homo sapiens (Human)
13773 CD226分子(CD226)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cluster Of Differentiation 226 (CD226) 48T/96T Homo sapiens (Human)
13774 兰尼定受体1(RYR1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Ryanodine Receptor 1, Skeletal (RYR1) 48T/96T Rattus norvegicus (Rat)
13775 甲状腺激素反应基因(THRSP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Thyroid Hormone Responsive (THRSP) 48T/96T Homo sapiens (Human)
13776 癌胚抗原相关细胞粘附分子8(CEACAM8)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8) 48T/96T Homo sapiens (Human)
13777 肌球蛋白重链8(MYH8)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8) 48T/96T Rattus norvegicus (Rat)
13778 尼克酰胺-N-甲基转移酶(NNMT)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Nicotinamide-N-Methyltransferase (NNMT) 48T/96T Mus musculus (Mouse)
13779 神经源素3(NEUROG3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Neurogenin 3 (NEUROG3) 48T/96T Mus musculus (Mouse)
13780 无翅型MMTV整合位点家族成员3A(WNT3A)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) 48T/96T Rattus norvegicus (Rat)
13781 10kDa干扰素γ诱导蛋白(IP10)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interferon Gamma Induced Protein 10kDa (IP10) 48T/96T Bos taurus; Bovine (Cattle)
13782 多聚免疫球蛋白受体(PIGR)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Polymeric Immunoglobulin Receptor (PIGR) 48T/96T Bos taurus; Bovine (Cattle)
13783 S100钙结合蛋白A8(S100A8)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for S100 Calcium Binding Protein A8 (S100A8) 48T/96T Bos taurus; Bovine (Cattle)
13784 S100钙结合蛋白A9(S100A9)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for S100 Calcium Binding Protein A9 (S100A9) 48T/96T Bos taurus; Bovine (Cattle)
13785 干扰素诱导T-细胞α亚族趋化剂(ITαC)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) 48T/96T Bos taurus; Bovine (Cattle)
13786 SATB同源框蛋白1(SATB1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for SATB Homeobox Protein 1 (SATB1) 48T/96T Homo sapiens (Human)
13787 乳酸脱氢酶C(LDHC)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Lactate Dehydrogenase C (LDHC) 48T/96T Homo sapiens (Human)
13788 CUB带状疮疹透明区样域蛋白1(CUZD1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for CUB And Zona Pellucida Like Domains Protein 1 (CUZD1) 48T/96T Homo sapiens (Human)
13789 猫眼综合征染色体区候选基因1(CECR1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1) 48T/96T Homo sapiens (Human)
13790 生长停滞特异性蛋白6(GAS6)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Growth Arrest Specific Protein 6 (GAS6) 48T/96T Homo sapiens (Human)
13791 信号素3E(SEMA3E)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Semaphorin 3E (SEMA3E) 48T/96T Homo sapiens (Human)
13792 抗*(AT)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Antithrombin (AT) 48T/96T Homo sapiens (Human)
13793 绒毛蛋白1(VIL1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Villin 1 (VIL1) 48T/96T Homo sapiens (Human)
13794 前列腺干细胞抗原(PSCA)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Prostate Stem Cell Antigen (PSCA) 48T/96T Homo sapiens (Human)
13795 前*α(PTMα)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Prothymosin Alpha (PTMa) 48T/96T Homo sapiens (Human)
13796 Kruppel样因子1(KLF1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Kruppel Like Factor 1, Erythroid (KLF1) 48T/96T Mus musculus (Mouse)
13797 钠/氢交换因子1(NHE1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Sodium/Hydrogen Exchanger 1 (NHE1) 48T/96T Homo sapiens (Human)
13798 不均一核核糖核蛋白K(HNRPK)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Heterogeneous Nuclear Ribonucleoprotein K (HNRPK) 48T/96T Homo sapiens (Human)
13799 甘油磷酸肌醇锚定高密度脂蛋白结合蛋白1(GPIHBP1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1) 48T/96T Homo sapiens (Human)
13800 胰岛素样蛋白3(INSL3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Insulin Like Protein 3 (INSL3) 48T/96T Mus musculus (Mouse)
13801 NEL样蛋白2(NELL2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for NEL Like Protein 2 (NELL2) 48T/96T Homo sapiens (Human)
13802 角蛋白14(KRT14)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Keratin 14 (KRT14) 48T/96T Homo sapiens (Human)
13803 *果糖-6-磷酸转氨酶2(GFPT2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Glutamine Fructose-6-Phosphate Transaminase 2 (GFPT2) 48T/96T Homo sapiens (Human)
13804 二肽基肽酶6(DPP6)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Dipeptidyl Peptidase 6 (DPP6) 48T/96T Homo sapiens (Human)
13805 反式高尔基体网状结构蛋白2(TGOLN2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Trans Golgi Network Protein 2 (TGOLN2) 48T/96T Homo sapiens (Human)
13806 内凝集蛋白2(ITLN2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Inectin 2 (ITLN2) 48T/96T Homo sapiens (Human)
13807 锌指同源框蛋白1B(ZFHX1B)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Zinc Finger Homeobox Protein 1B (ZFHX1B) 48T/96T Mus musculus (Mouse)
13808 DnaJ/Hsp40同源物亚家族C成员12(DNAJC12)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for DnaJ/HSP40 Homolog Subfamily C, Member 12 (DNAJC12) 48T/96T Homo sapiens (Human)
13809 杀伤细胞免疫球蛋白样受体3DL1(KIR3DL1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1) 48T/96T Homo sapiens (Human)
13810 节律昼夜蛋白1(PER1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Period Circadian Protein 1 (PER1) 48T/96T Homo sapiens (Human)
13811 Clock同源物(CLOCK)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Clock Homolog (CLOCK) 48T/96T Homo sapiens (Human)
13812 转胶蛋白(TAGLN)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Transgelin (TAGLN) 48T/96T Homo sapiens (Human)
13813 隐花色素1(CRY1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cryptochrome 1 (CRY1) 48T/96T Homo sapiens (Human)
13814 配对框基因9(PAX9)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Paired Box Gene 9 (PAX9) 48T/96T Homo sapiens (Human)
13815 过氧化还原酶4(PRDX4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Peroxiredoxin 4 (PRDX4) 48T/96T Mus musculus (Mouse)
13816 X-射线修复交叉互补蛋白6(XRCC6)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for X-Ray Repair Cross Complementing 6 (XRCC6) 48T/96T Mus musculus (Mouse)
13817 DNA修复蛋白RAD50(RAD50)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for DNA Repair Protein RAD50 (RAD50) 48T/96T Mus musculus (Mouse)
13818 髓细胞触发受体1(IREM1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Immune Receptor Expressed On Myeloid Cells 1 (IREM1) 48T/96T Homo sapiens (Human)
13819 印度刺猬因子(IHH)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Hedgehog Homolog, Indian (IHH) 48T/96T Mus musculus (Mouse)
13820 POTE锚蛋白域家族成员G(POTEG)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for POTE Ankyrin Domain Family, Member G (POTEG) 48T/96T Homo sapiens (Human)
13821 Neudesin神经营养因子(NENF)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Neudesin Neurotrophic Factor (NENF) 48T/96T Homo sapiens (Human)
13822 细胞色素P450家族成员7A1(CYP7A1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cytochrome P450 7A1 (CYP7A1) 48T/96T Mus musculus (Mouse)
13823 高迁移率族AT Hook蛋白1(HMGA1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for High Mobility Group AT Hook Protein 1 (HMGA1) 48T/96T Canis familiaris; Canine (Dog)
13824 白介素3受体α(IL3Rα)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interleukin 3 Receptor Alpha (IL3Ra) 48T/96T Homo sapiens (Human)
13825 神经肽Y受体Y1(NPY1R)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Neuropeptide Y Receptor Y1 (NPY1R) 48T/96T Rattus norvegicus (Rat)
13826 神经肽Y受体Y2(NPY2R)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Neuropeptide Y Receptor Y2 (NPY2R) 48T/96T Rattus norvegicus (Rat)
13827 白介素17D(IL17D)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interleukin 17D (IL17D) 48T/96T Homo sapiens (Human)
13828 肽基*脱亚氨酶Ⅳ(PADI4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Peptidyl Arginine Deiminase Type IV (PADI4) 48T/96T Homo sapiens (Human)
13829 基质金属蛋白酶16(MMP16)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Matrix Metalloproteinase 16 (MMP16) 48T/96T Homo sapiens (Human)
13830 封闭蛋白4(CLDN4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Claudin 4 (CLDN4) 48T/96T Rattus norvegicus (Rat)
13831 高尔基蛋白73(GP73)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Golgi Protein 73 (GP73) 48T/96T Mus musculus (Mouse)
13832 信号素4D(SEMA4D)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Semaphorin 4D (SEMA4D) 48T/96T Homo sapiens (Human)
13833 信号淋巴细胞激活分子家族成员7(SLAMF7)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Signaling Lymphocytic Activation Molecule Family, Member 7 (SLAMF7) 48T/96T人乳腺癌*耐药株,MCF ADR细胞Homo sapiens (Human)
13834 丛状蛋白B1(PLXNB1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Plexin B1 (PLXNB1) 48T/96T Homo sapiens (Human)